ArticlePloS one2009
Spectral unmixing: analysis of performance in the olfactory bulb in vivo.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
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- Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.Brain structure & function · 2018Review
- Design and implementation of multi-signal and time-varying neural reconstructions.Scientific data · 2018Article
- Detection of phasic dopamine by D1 and D2 striatal medium spiny neurons.The Journal of physiology · 2017Article
- Real-time intravital imaging of pH variation associated with osteoclast activity.Nature chemical biology · 2016Article
- Snapshot Hyperspectral Volumetric Microscopy.Scientific reports · 2016Article
- Intravital FRET: Probing Cellular and Tissue Function in Vivo.International journal of molecular sciences · 2015Article
- Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser.Optics express · 2013Article
- Striatal neurones have a specific ability to respond to phasic dopamine release.The Journal of physiology · 2013Article
- Analysis of targeted viral protein nanoparticles delivered to HER2+ tumors.Journal of visualized experiments : JoVE · 2013Article
- Parallelized TCSPC for dynamic intravital fluorescence lifetime imaging: quantifying neuronal dysfunction in neuroinflammation.PloS one · 2013Article
- Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging.Nature protocols · 2011Article
- In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice.American journal of physiology. Heart and circulatory physiology · 2010Article
- Simultaneous live cell imaging using dual FRET sensors with a single excitation light.PloS one · 2009Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe generation of transgenic mice expressing combinations of fluorescent proteins has greatly aided the reporting of activity and identification of specific neuronal populations. Methods capable of separating multiple overlapping fluorescence emission spectra, deep in the living brain, with high sensitivity and temporal resolution are therefore required. Here, we investigate to what extent spectral unmixing addresses these issues. METHODOLOGY/PRINCIPAL
findingsUsing fluorescence resonance energy transfer (FRET)-based reporters, and two-photon laser scanning microscopy with synchronous multichannel detection, we report that spectral unmixing consistently improved FRET signal amplitude, both in vitro and in vivo. Our approach allows us to detect odor-evoked FRET transients 180-250 microm deep in the brain, the first demonstration of in vivo spectral imaging and unmixing of FRET signals at depths greater than a few tens of micrometer. Furthermore, we determine the reporter efficiency threshold for which FRET detection is improved by spectral unmixing. CONCLUSIONS/SIGNIFICANCE: Our method allows the detection of small spectral variations in depth in the living brain, which is essential for imaging efficiently transgenic animals expressing combination of multiple fluorescent proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.