ArticleEplasty2009
A human full-skin culture system for interventional studies.
Article in Eplasty, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- Skin Deep: Uncovering the Early Events of Crimean-Congo Hemorrhagic Fever Virus at the Tick-Host-Virus Interface.Viruses · 2026Review
- A human ex vivo skin model breaking boundaries.Scientific reports · 2024Article
- Human Skin Explant Preparation and Culture.Bio-protocol · 2022Article
- Inflammatory and coagulative pathophysiology for the management of burn patients with COVID-19: systematic review of the evidence.Annals of burns and fire disasters · 2021Article
- Human skin explant model for the investigation of topical therapeutics.Scientific reports · 2020Article
- Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants.International journal of molecular sciences · 2020Article
- Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Lipid functions in skin: Differential effects of n-3 polyunsaturated fatty acids on cutaneous ceramides, in a human skin organ culture model.Biochimica et biophysica acta. Biomembranes · 2017Article
- Bioluminescence-mediated longitudinal monitoring of adipose-derived stem cells in a large mammal ex vivo organ culture.Scientific reports · 2015Article
- Usefulness of Skin Explants for Histologic Analysis after Fractional Photothermolysis.Annals of dermatology · 2015Article
- Innate immune response after adenoviral gene delivery into skin is mediated by AIM2, NALP3, DAI and mda5.SpringerPlus · 2013Article
- Evaluation of signal transduction pathways after transient cutaneous adenoviral gene delivery.BMC immunology · 2011Article
- A novel human skin chamber model to study wound infection ex vivo.Archives of dermatological research · 2010Article
- A skin explant model for studying UV-induced DNA damage and repair.Photochemistry and photobiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveNovel approaches to bridge the gap between clinical studies and experimental basic research of skin physiology are urgently needed. The aim of this study was to develop an effective surrogate model in which ex vivo full-thickness organ culture experiments may be performed.
methodsHuman full skin from patients was placed into a stainless steel chamber and cultured at an air-liquid interphase for 4 weeks. Samples were evaluated every week by HE-staining and immunohistochemical characterization. Epidermal gene transfer kinetics was performed as an interventional study.
resultsThis ex vivo chamber model maintained the physiologic and histologic properties of the skin explants for 4 weeks. This indicated the model's acceptable ex vivo physiologic validity. No epidermolysis was observed, and both basal lamina and blood vessels were detected within all tissue samples. Transgene expression was demonstrated to be time dependent.
conclusionThis model chamber presents a convenient, easy-to-use, and robust model in which ex vivo full-thickness organ culture experiments may be performed.
Identifiers
19198642PMC2627306What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.