ArticleBiotechnology progress
Scalable culture and cryopreservation of human embryonic stem cells on microcarriers.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Cryogenic microcarrier-assisted stem cell storage (Cryo-MASCS): a preservation method enabling scalable culture of human mesenchymal stem cells.Stem cell research & therapy · 2025Article
- Suspension culture on microcarriers and as aggregates enables expansion and differentiation of pluripotent stem cells (PSCs).Cytotherapy · 2023Article
- Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering.International journal of molecular sciences · 2023Article
- A Review of the Use of Microparticles for Cartilage Tissue Engineering.International journal of molecular sciences · 2021Review
- End-to-End Platform for Human Pluripotent Stem Cell Manufacturing.International journal of molecular sciences · 2019Article
- AFM nanoindentation reveals decrease of elastic modulus of lipid bilayers near freezing point of water.Scientific reports · 2019Article
- A novel tool for suspension culture of human induced pluripotent stem cells: Lysophospholipids as a cell aggregation regulator.Regenerative therapy · 2019Article
- Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term DynamicTissue engineering and regenerative medicine · 2019Article
- A fully defined static suspension culture system for large-scale human embryonic stem cell production.Cell death & disease · 2018Article
- Review
- Engineering Xeno-Free Microcarriers with Recombinant Vitronectin, Albumin and UV Irradiation for Human Pluripotent Stem Cell Bioprocessing.ACS biomaterials science & engineering · 2017Article
- Three-Dimensional Hyaluronic Acid Hydrogel-Based Models for In Vitro Human iPSC-Derived NPC Culture and Differentiation.Journal of materials chemistry. B · 2017Article
- Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.Scientific reports · 2017Article
- Efficient generation of hPSC-derived midbrain dopaminergic neurons in a fully defined, scalable, 3D biomaterial platform.Scientific reports · 2017Article
- Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells.Cytotechnology · 2016Article
- Article
- Enzymatically degradable poly(ethylene glycol) hydrogels for the 3D culture and release of human embryonic stem cell derived pancreatic precursor cell aggregates.Acta biomaterialia · 2015Article
- Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems.Reproductive biology and endocrinology : RB&E · 2015Review
- Production of human pluripotent stem cell therapeutics under defined xeno-free conditions: progress and challenges.Stem cell reviews and reports · 2015Review
- Cationic surface charge combined with either vitronectin or laminin dictates the evolution of human embryonic stem cells/microcarrier aggregates and cell growth in agitated cultures.Stem cells and development · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
As a result of their pluripotency and potential for unlimited self-renewal, human embryonic stem cells (hESCs) hold tremendous promise in regenerative medicine. An essential prerequisite for the widespread application of hESCs is the establishment of effective and efficient protocols for large-scale cell culture, storage, and distribution. At laboratory scales hESCs are cultured adherent to tissue culture plates; these culture techniques are labor-intensive and do not scale to high cell numbers. In an effort to facilitate larger scale hESC cultivation, we investigated the feasibility of culturing hESCs adherent to microcarriers. We modified the surface of Cytodex 3 microcarriers with either Matrigel or mouse embryonic fibroblasts (MEFs). hESC colonies were effectively expanded in a pluripotent, undifferentiated state on both Matrigel-coated microcarriers and microcarriers seeded with a MEF monolayer. While the hESC expansion rate on MEF-microcarriers was less than that on MEF-plates, the doubling time of hESCs on Matrigel-microcarriers was indistinguishable from that of hESCs expanded on Matrigel-coated tissue culture plates. Standard hESC cryopreservation methodologies are plagued by poor viability and high differentiation rates upon thawing. Here, we demonstrate that cryopreservation of hESCs adherent to microcarriers in cryovials provides a higher recovery of undifferentiated cells than cryopreservation of cells in suspension. Together, these results suggest that microcarrier-based stabilization and culture may facilitate hESC expansion and storage for research and therapeutic applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.