ArticleEnvironmental and molecular mutagenesis2009
Requirement of the Saccharomyces cerevisiae APN1 gene for the repair of mitochondrial DNA alkylation damage.
Article in Environmental and molecular mutagenesis, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- A system for inducible mitochondria-specific protein degradation in vivo.Nature communications · 2024Article
- Dual targeting of Saccharomyces cerevisiae Pso2 to mitochondria and the nucleus, and its functional relevance in the repair of DNA interstrand crosslinks.G3 (Bethesda, Md.) · 2022Article
- DNA Repair in Haploid Context.International journal of molecular sciences · 2021Review
- Identifying novel protein phenotype annotations by hybridizing protein-protein interactions and protein sequence similarities.Molecular genetics and genomics : MGG · 2016Article
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- Detection of DNA damage in peripheral blood mononuclear cells from pancreatic cancer patients.Molecular carcinogenesis · 2015Article
- MMS exposure promotes increased MtDNA mutagenesis in the presence of replication-defective disease-associated DNA polymerase γ variants.PLoS genetics · 2014Article
- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Review
- Apn1 AP-endonuclease is essential for the repair of oxidatively damaged DNA bases in yeast frataxin-deficient cells.Human molecular genetics · 2012Article
- Oxidants and not alkylating agents induce rapid mtDNA loss and mitochondrial dysfunction.DNA repair · 2012Article
- Aging increases mitochondrial DNA damage and oxidative stress in liver of rhesus monkeys.Experimental gerontology · 2012Article
- Mitochondrial DNA, chloroplast DNA and the origins of development in eukaryotic organisms.Biology direct · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae APN1 gene that participates in base excision repair has been localized both in the nucleus and the mitochondria. APN1 deficient cells (apn1 Delta) show increased mutation frequencies in mitochondrial DNA (mtDNA) suggesting that APN1 is also important for mtDNA stability. To understand APN1-dependent mtDNA repair processes we studied the formation and repair of mtDNA lesions in cells exposed to methyl methanesulfonate (MMS). We show that MMS induces mtDNA damage in a dose-dependent fashion and that deletion of the APN1 gene enhances the susceptibility of mtDNA to MMS. Repair kinetic experiments demonstrate that in wild-type cells (WT) it takes 4 hr to repair the damage induced by 0.1% MMS, whereas in the apn1 Delta strain there is a lag in mtDNA repair that results in significant differences in the repair capacity between the two yeast strains. Analysis of lesions in nuclear DNA (nDNA) after treatment with 0.1% MMS shows a significant difference in the amount of nDNA lesions between WT and apn1 Delta cells. Interestingly, comparisons between nDNA and mtDNA damage show that nDNA is more sensitive to the effects of MMS treatment. However, both strains are able to repair the nDNA lesions, contrary to mtDNA repair, which is compromised in the apn1 Delta mutant strain. Therefore, although nDNA is more sensitive than mtDNA to the effects of MMS, deletion of APN1 has a stronger phenotype in mtDNA repair than in nDNA. These results highlight the prominent role of APN1 in the repair of environmentally induced mtDNA damage.
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