ArticlePloS one2009
The fetal hypothalamus has the potential to generate cells with a gonadotropin releasing hormone (GnRH) phenotype.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- An optimized method for directed differentiation of hypothalamic neural stem cells in a 3D culture system.Scientific reports · 2025Article
- An Efficient Method for Generating Murine Hypothalamic Neurospheres for the Study of Regional Neural Progenitor Biology.Endocrinology · 2020Article
- A comparison of the sexually dimorphic dexamethasone transcriptome in mouse cerebral cortical and hypothalamic embryonic neural stem cells.Molecular and cellular endocrinology · 2018Article
- Development of Gonadotropin-Releasing Hormone-Secreting Neurons from Human Pluripotent Stem Cells.Stem cell reports · 2016Article
- Research Resource: The Dexamethasone Transcriptome in Hypothalamic Embryonic Neural Stem Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons.Biology open · 2015Article
- Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells.Neurogenesis (Austin, Tex.) · 2014Article
- Neurodevelopmental origin and adult neurogenesis of the neuroendocrine hypothalamus.Frontiers in cellular neuroscience · 2014Review
- Article
- Proliferative hypothalamic neurospheres express NPY, AGRP, POMC, CART and Orexin-A and differentiate to functional neurons.PloS one · 2011Article
- Fibroblast growth factor signaling in the developing neuroendocrine hypothalamus.Frontiers in neuroendocrinology · 2011Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNeurospheres (NS) are colonies of neural stem and precursor cells capable of differentiating into the central nervous system (CNS) cell lineages upon appropriate culture conditions: neurons, and glial cells. NS were originally derived from the embryonic and adult mouse striatum subventricular zone. More recently, experimental evidence substantiated the isolation of NS from almost any region of the CNS, including the hypothalamus. METHODOLOGY/
findingsHere we report a protocol that enables to generate large quantities of NS from both fetal and adult rat hypothalami. We found that either FGF-2 or EGF were capable of inducing NS formation from fetal hypothalamic cultures, but that only FGF-2 is effective in the adult cultures. The hypothalamic-derived NS are capable of differentiating into neurons and glial cells and most notably, as demonstrated by immunocytochemical detection with a specific anti-GnRH antibody, the fetal cultures contain cells that exhibit a GnRH phenotype upon differentiation. CONCLUSIONS/SIGNIFICANCE: This in vitro model should be useful to study the molecular mechanisms involved in GnRH neuronal differentiation.
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