ArticleThe Journal of molecular diagnostics : JMD2009
LightCycler technology in molecular diagnostics.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Quantitative DNA melting analysis with hybridization probes (qDMA-HP) as a novel approach to assess MGMT promoter methylation in malignant glioma.Journal of neuro-oncology · 2026Article
- Fluorescent Probes for the Quantification of RNA by Real-Time qPCR.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Atypical Presentations and Molecular Diagnosis of Ocular Bartonellosis.International journal of molecular sciences · 2025Review
- Genetic Prognostic Factors in Adult Diffuse Gliomas: A 10-Year Experience at a Single Institution.Cancers · 2024Article
- Semiautomated Electrochemical Melting Curve Analysis Device for the Detection of an Osteoporosis Associated Single Nucleotide Polymorphism in Blood.Analytical chemistry · 2023Article
- Rapid Cycle and Extreme Polymerase Chain Reaction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Experimental infection of cats withJournal of feline medicine and surgery · 2022Article
- Human genetic variant E756del in the ion channel PIEZO1 not associated with protection from severe malaria in a large Ghanaian study.Journal of human genetics · 2022Article
- Extraction-Free Rapid Cycle Quantitative RT-PCR and Extreme RT-PCR for SARS-CoV-2 Virus Detection.The Journal of molecular diagnostics : JMD · 2021Article
- Rapid screening ofExperimental and therapeutic medicine · 2021Article
- Cell-blocks and other ancillary studies (including molecular genetic tests and proteomics).CytoJournal · 2021Review
- Quadruplex real-time PCR for rapid detection of human alphaherpesviruses.Medical microbiology and immunology · 2019Article
- The kinetic requirements of extreme qPCR.Biomolecular detection and quantification · 2019Article
- Rapid detection of functional gene polymorphisms of TLRs and IL-17 using high resolution melting analysis.Scientific reports · 2017Article
- MnOChemical science · 2017Article
- NRAS and EPHB6 mutation rates differ in metastatic melanomas of patients in the North Island versus South Island of New Zealand.Oncotarget · 2016Article
- Rapid Optical Cavity PCR.Advanced healthcare materials · 2016Article
- Article
- Enhanced specificity of TPMT*2 genotyping using unidirectional wild-type and mutant allele-specific scorpion primers in a single tube.PloS one · 2014Article
- Early detection of Trichinella spiralis in muscle of infected mice by real-time fluorescence resonance energy transfer PCR.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
LightCycler technology combines rapid-cycle polymerase chain reaction with real-time fluorescent monitoring and melting curve analysis. Since its introduction in 1997, it is now used in many areas of molecular pathology, including oncology (solid tumors and hematopathology), inherited disease, and infectious disease. By monitoring product accumulation during rapid amplification, quantitative polymerase chain reaction in a closed-tube system is possible in 15 to 30 minutes. Furthermore, melting curve analysis of probes and/or amplicons provides genotyping and even haplotyping. Novel mutations are identified by unexpected melting temperature or curve shape changes. Melting probe designs include adjacent hybridization probes, single labeled probes, unlabeled probes, and snapback primers. High-resolution melting allows mutation scanning by detecting all heterozygous changes. This review describes the major advances throughout the last 15 years regarding LightCycler technology and its application in clinical laboratories.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.