ArticleProceedings of the National Academy of Sciences of the United States of America2009
Drosophila hnRNP A1 homologs Hrp36/Hrp38 enhance U2-type versus U12-type splicing to regulate alternative splicing of the prospero twintron.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The minor spliceosome component U4atac regulates JAK/STAT signaling to modulate hematopoiesis and immune responses in Drosophila melanogaster.Nature communications · 2026Article
- Multifunctional Non-Coding RNAs Mediate Latent Infection and Recurrence of Herpes Simplex Viruses.Infection and drug resistance · 2021Review
- At the Intersection of Major and Minor Spliceosomes: Crosstalk Mechanisms and Their Impact on Gene Expression.Frontiers in genetics · 2021Review
- Transcriptional Regulation of Latency-Associated Transcripts (LATs) of Herpes Simplex Viruses.Journal of Cancer · 2020Article
- A mechanism for a single nucleotide intron shift.Nucleic acids research · 2017Article
- Poly(ADP-Ribosyl)ation of hnRNP A1 Protein Controls Translational Repression in Drosophila.Molecular and cellular biology · 2016Article
- Charon Mediates Immune Deficiency-Driven PARP-1-Dependent Immune Responses in Drosophila.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- The Drosophila Helicase Maleless (MLE) is Implicated in Functions Distinct From its Role in Dosage Compensation.Molecular & cellular proteomics : MCP · 2015Article
- Review
- Emerging roles for hnRNPs in post-transcriptional regulation: what can we learn from flies?Chromosoma · 2014Review
- A day in the life of the spliceosome.Nature reviews. Molecular cell biology · 2014Review
- Article
- The half-life of the HSV-1 1.5-kb LAT intron is similar to the half-life of the 2.0-kb LAT intron.Journal of neurovirology · 2013Article
- AUF1/hnRNP D is a novel protein partner of the EBER1 noncoding RNA of Epstein-Barr virus.RNA (New York, N.Y.) · 2012Article
- The hnRNP A1 homolog Hrp36 is essential for normal development, female fecundity, omega speckle formation and stress tolerance in Drosophila melanogaster.Journal of biosciences · 2012Article
- Poly(ADP-ribose) controls DE-cadherin-dependent stem cell maintenance and oocyte localization.Nature communications · 2012Article
- Regulated functional alternative splicing in Drosophila.Nucleic acids research · 2012Review
- In vivo effects of traditional Ayurvedic formulations in Drosophila melanogaster model relate with therapeutic applications.PloS one · 2012Article
- U12-type spliceosomal introns of Insecta.International journal of biological sciences · 2012Article
- Sqd interacts with the Drosophila retinoblastoma tumor suppressor Rbf.Biochemical and biophysical research communications · 2009Article
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3 authors.
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Abstract
During Drosophila embryogenesis, the transcription factor Prospero is critical for neuronal differentiation and axonal outgrowth. The prospero pre-mRNA undergoes alternative splicing, but is unique in that it harbors a rare twintron whereby one intron lies embedded within another. The innermost intron is excised by the major U2-type spliceosome and the outermost is excised by the minor U12-type spliceosome. Previously, an intronic purine-rich element (PRE) was identified as an enhancer of both U2- and U12-type splicing, with a greater effect on the U2-type pathway. We find that the PRE binds Drosophila homologs of heterogeneous nuclear ribonucleoprotein (hnRNP) A1, Hrp38 and Hrp36. RNAi-mediated knockdown of these proteins in S2 cells specifically decreases U2-type splicing of the twintron, which is surprising because hnRNPs usually are repressive. Conversely, tethering Hrp38 to the twintron increases U2-type splicing. Thus, developmentally regulated alternative splicing of the prospero twintron can be explained by documented changes in the abundance of these hnRNP A1-like proteins during embryogenesis.
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