ArticleBMC genomics2009
Understanding Haemophilus parasuis infection in porcine spleen through a transcriptomics approach.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
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- Identification and differential expression analysis of microRNAs in the liver and spleen tissues of Yunnan Zebu and Holstein cattle.Tropical animal health and production · 2025Article
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- Resistin secreted by porcine alveolar macrophages leads to endothelial cell dysfunction duringVirulence · 2023Article
- Changes in salivary biomarkers of stress, inflammation, redox status, and muscle damage due to Streptococcus suis infection in pigs.BMC veterinary research · 2023Article
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- Comparison of theFrontiers in veterinary science · 2021Article
- Selection and validation of differentially expressed metabolic and immune genes in weaned Ghurrah versus crossbred piglets.Tropical animal health and production · 2020Article
- Comparative Analysis of the Liver and Spleen Transcriptomes between Holstein and Yunnan Humped Cattle.Animals : an open access journal from MDPI · 2019Article
- The Multiple Faces of the Metal Transporter ZIP14 (SLC39A14).The Journal of nutrition · 2018Review
- Transcriptional Profiling of Leucocyte Count Variation from Porcine Peripheral Blood Reveals Differential Gene Expression.BioMed research international · 2018Article
- Genome editing in livestock: Are we ready for a revolution in animal breeding industry?Transgenic research · 2017Review
- RNA Seq analysis for transcriptome profiling in response to classical swine fever vaccination in indigenous and crossbred pigs.Functional & integrative genomics · 2017Article
- Genome-wide analysis of the transcriptional response to porcine reproductive and respiratory syndrome virus infection at the maternal/fetal interface and in the fetus.BMC genomics · 2016Article
- Current transcriptomics in pig immunity research.Mammalian genome : official journal of the International Mammalian Genome Society · 2015Review
- Breed-specific transcriptome response of spleen from six to eight week old piglet after infection with Streptococcus suis type 2.Molecular biology reports · 2014Article
- Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets.BMC genomics · 2014Article
- The inflammation-related gene S100A12 is positively regulated by C/EBPβ and AP-1 in pigs.International journal of molecular sciences · 2014Article
- Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2.BMC genomics · 2013Article
- Structural and functional annotation of the porcine immunome.BMC genomics · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHaemophilus parasuis (HPS) is an important swine pathogen that causes Glässer's disease, which is characterized by fibrinous polyserositis, meningitis and arthritis. The molecular mechanisms that underlie the pathogenesis of the disease remain poorly understood, particularly the resistance of porcine immune system to HPS invasion. In this study, we investigated the global changes in gene expression in the spleen following HPS infection using the Affymetrix Porcine Genechip.
resultsA total of 931 differentially expressed (DE) transcripts were identified in the porcine spleen 7 days after HPS infection; of these, 92 unique genes showed differential expression patterns based on analysis using BLASTX and Gene Ontology. The DE genes involved in the immune response included genes for inflammasomes (RETN, S100A8, S100A9, S100A12), adhesion molecules (CLDN3, CSPG2, CD44, LGALS8), transcription factors (ZBTB16, SLC39A14, CEBPD, CEBPB), acute-phase proteins and complement (SAA1, LTF, HP, C3), differentiation genes for epithelial cells and keratinocytes (TGM1, MS4A8B, CSTA), and genes related to antigen processing and presentation (HLA-B, HLA-DRB1). Further immunostimulation analyses indicated that mRNA levels of S100A8, S100A9, and S100A12 in porcine PK-15 cells increased within 48 h and were sustained after administration of lipopolysaccharide (LPS) and Poly(I:C) respectively. In addition, mapping of DE genes to porcine health traits QTL regions showed that 70 genes were distributed in 7 different known porcine QTL regions. Finally, 10 DE genes were validated by quantitative PCR.
conclusionOur findings demonstrate previously unrecognized changes in gene transcription that are associated with HPS infection in vivo, and many potential cascades identified in the study clearly merit further investigation. Our data provide new clues to the nature of the immune response in mammals, and we have identified candidate genes that are related to resistance to HPS.
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