ArticleStem cell research2007
Effects of fgf2 and oxygen in the bmp4-driven differentiation of trophoblast from human embryonic stem cells.
Article in Stem cell research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 91 citations in OpenAlex.
- Synthetic embryos: current progress, application and challenges.Reproductive biology and endocrinology : RB&E · 2026Review
- Emerging models of human and non-human primate placental development - Centre for Trophoblast Research 17th annual meeting 2024.Biology open · 2024Review
- ISRIB facilitates the co-culture of human trophoblast stem cells and embryonic stem cells.Cell proliferation · 2024Article
- iPSC-based modeling of preeclampsia identifies epigenetic defects in extravillous trophoblast differentiation.iScience · 2024Article
- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.Current protocols · 2023Article
- Article
- Exploring maternal-fetal interface with in vitro placental and trophoblastic models.Frontiers in cell and developmental biology · 2023Review
- The role of BMP4 signaling in trophoblast emergence from pluripotency.Cellular and molecular life sciences : CMLS · 2022Review
- Polycomb repressive complex 2 shields naïve human pluripotent cells from trophectoderm differentiation.Nature cell biology · 2022Article
- BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo.Biology open · 2021Article
- Modeling human peri-implantation placental development and function†.Biology of reproduction · 2021Review
- Modeling preeclampsia using human induced pluripotent stem cells.Scientific reports · 2021Article
- Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State.Stem cells international · 2021Article
- Single Nucleus RNA Sequence (snRNAseq) Analysis of the Spectrum of Trophoblast Lineages Generated From Human Pluripotent Stem CellsFrontiers in cell and developmental biology · 2021Article
- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- NLRP7 plays a functional role in regulating BMP4 signaling during differentiation of patient-derived trophoblasts.Cell death & disease · 2020Article
- Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Modeling human trophoblast, the placental epithelium at the maternal fetal interface.Reproduction (Cambridge, England) · 2020Review
- Use of a human embryonic stem cell model to discover GABRP, WFDC2, VTCN1 and ACTC1 as markers of early first trimester human trophoblast.Molecular human reproduction · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Human embryonic stem cells (hESC) differentiate into trophoblast when treated with BMP4. Here we studied the effects of either low (4 % O(2), L) or atmospheric O(2) (20% O(2), A) in the presence and absence of FGF2 on H1 hESC cultured in presence of BMP4. Differentiation progressed from the periphery towards the center of colonies. It occurred most quickly in the absence of FGF2 and under A and was slowest in presence of FGF2 and under L. Chorionic gonadotrophin (CG) production required A while FGF2 suppressed progesterone synthesis under both A and L. FGF2 was then omitted while we examined trophoblast markers SSEA-1 and cytokeratin-7 and -8, whose expression also progressed inwards from the periphery of colonies and occurred more rapidly under A than L. By day 5, most cells outside central islands of Oct4-positive cells were positive for these antigens under both conditions and many also expressed HLA-G, a marker of extra-villous cytotrophoblast. Under A, but not L, CGalpha and CGbeta became prominent in GATA2-positive, peripherally located, multinucleated cells. In conclusion, BMP4 induced conversion of hESC exclusively towards trophoblast; FGF2 slowed differentiation, while O(2) accelerated this process and promoted syncytiotrophoblast formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.