ArticleAmerican journal of physiology. Cell physiology2009
Attenuation of proliferation and migration of retinal pericytes in the absence of thrombospondin-1.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 73 citations in OpenAlex.
- Single Cell RNA Sequencing Reveals THBS1+CD14+ Monocyte Modulates Inflammatory Activation via NRLP3-Inflammasome in Congenital Heart Block.Journal of cellular and molecular medicine · 2026Article
- TSP-1, TSP-2, and TSP-5 demonstrate sexual dimorphism in intimal hyperplasia in rats and mice.American journal of physiology. Heart and circulatory physiology · 2025Article
- Isolation and Characterization of Mouse Choroidal Melanocytes and Their Proinflammatory Characteristics.Cells · 2025Article
- Membrane Ruffles: Composition, Function, Formation and Visualization.International journal of molecular sciences · 2024Review
- Thrombospondin-1 in vascular development, vascular function, and vascular disease.Seminars in cell & developmental biology · 2024Review
- Retinal inflammation in murine models of type 1 and type 2 diabetes with diabetic retinopathy.Diabetologia · 2023Article
- Article
- Exosomes derived from LPS-stimulated human thymic mesenchymal stromal cells enhance inflammation via thrombospondin-1.Bioscience reports · 2021Article
- Therapeutic effects of placenta derived-, umbilical cord derived-, and adipose tissue derived-mesenchymal stem cells in chronicJournal of clinical biochemistry and nutrition · 2021Article
- Caffeine Inhibits Choroidal Neovascularization Through Mitigation of Inflammatory and Angiogenesis Activities.Frontiers in cell and developmental biology · 2021Article
- Targeted Thrombospondin-1 Expression in Ocular Vascular Development and Neovascularization.Frontiers in cell and developmental biology · 2021Article
- Noninvasive temporal detection of early retinal vascular changes during diabetes.Scientific reports · 2020Article
- 1,25(OH)FASEB bioAdvances · 2019Article
- Targeting pericytes for neurovascular regeneration.Cell communication and signaling : CCS · 2019Review
- Time-lapse microscopy of oxidative stress demonstrates metabolic sensitivity of retinal pericytes under high glucose condition.Journal of biophotonics · 2018Article
- PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells.American journal of physiology. Cell physiology · 2018Article
- Mice dental pulp and periodontal ligament endothelial cells exhibit different proangiogenic properties.Tissue & cell · 2018Article
- Agent-based computational model of retinal angiogenesis simulates microvascular network morphology as a function of pericyte coverage.Microcirculation (New York, N.Y. : 1994) · 2017Article
- Negative regulators of angiogenesis: important targets for treatment of exudative AMD.Clinical science (London, England : 1979) · 2017Review
- β2-Adrenergic Receptor Antagonism Attenuates CNV Through Inhibition of VEGF and IL-6 Expression.Investigative ophthalmology & visual science · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Perivascular supporting cells, including vascular smooth muscle cells (VSMCs) and pericytes (PCs), provide instructive signals to adjacent endothelial cells helping to maintain vascular homeostasis. These signals are provided through direct contact and by the release of soluble factors by these cells. Thrombospondin (TSP)1 is a matricellular protein and an autocrine factor for VSMCs. TSP1 activity, along with that of PDGF, regulates VSMC proliferation and migration. However, the manner in which TSP1 and PDGF impact retinal PC function requires further investigation. In the present study, we describe, for the first time, the isolation and culture of retinal PCs from wild-type (TSP1(+/+)) and TSP1-deficient (TSP1(-/-)) immortomice. We showed that these cells express early and mature markers of PCs, including NG2, PDGF receptor-beta, and smooth muscle actin as well as desmin, calbindin, and mesenchymal stem cell markers. These cells were successfully passaged and maintained in culture for several months without significant loss of expression of these markers. TSP1(+/+) PCs proliferated at a faster rate compared with TSP1(-/-) PCs. In addition, TSP1(+/+) PCs, like VSMCs, responded to PDGF-BB with enhanced migration and proliferation. In contrast, TSP1(-/-) PCs failed to respond to the promigratory and proliferative activity of PDGF-BB. This may be attributed, at least in part, to the limited interaction of PDGF-BB with TSP1 in null cells, which is essential for PDGF proliferative and migratory action. We observed no significant differences in the rates of apoptosis in these cells. TSP1(-/-) PCs were also less adherent, expressed increased levels of TSP2 and fibronectin, and had decreased amounts of N-cadherin and alpha(v)beta(3)-integrin on their surface. Thus, TSP1 plays a significant role in retinal PC proliferation and migration impacting retinal vascular development and homeostasis.
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