ArticleJournal of clinical microbiology2009
Identification of clinically relevant nonhemolytic Streptococci on the basis of sequence analysis of 16S-23S intergenic spacer region and partial gdh gene.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Identification of Clinically RelevantPathogens (Basel, Switzerland) · 2020Article
- Species-Specific Identification of Streptococcus based on DNA Marker in 16S-23S rDNA Internal Transcribed Spacer.Current microbiology · 2020Article
- Pseudomonas aeruginosa Can Inhibit Growth of Streptococcal Species via Siderophore Production.Journal of bacteriology · 2019Article
- Current Challenges ofMolecules and cells · 2018Review
- Whole genome sequencing as a tool for phylogenetic analysis of clinical strains of Mitis group streptococci.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- A Genome-Wide Profiling Strategy as an Aid for Searching Unique Identification Biomarkers for Streptococcus.Indian journal of microbiology · 2016Article
- Using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Complemented with Selected 16S rRNA and gyrB Genes Sequencing to Practically Identify Clinical Important Viridans Group Streptococci (VGS).Frontiers in microbiology · 2016Article
- The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans.BMC microbiology · 2014Article
- GyrB polymorphisms accurately assign invasive viridans group streptococcal species.Journal of clinical microbiology · 2014Article
- Advantages and limitations of ribosomal RNA PCR and DNA sequencing for identification of bacteria in cardiac valves of danish patients.The open microbiology journal · 2013Article
- Novel molecular method for identification of Streptococcus pneumoniae applicable to clinical microbiology and 16S rRNA sequence-based microbiome studies.Journal of clinical microbiology · 2012Article
- Oral streptococcal bacteremia in hospitalized patients: taxonomic identification and clinical characterization.Journal of clinical microbiology · 2012Article
- Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus.Annals of clinical microbiology and antimicrobials · 2011Article
- recA-based PCR assay for accurate differentiation of Streptococcus pneumoniae from other viridans streptococci.Journal of clinical microbiology · 2011Article
- Species diversity and relative abundance of lactic acid bacteria in the milk of rhesus monkeys (Macaca mulatta).Journal of medical primatology · 2011Article
- Article
- Infective Endocarditis: Identification of Catalase-Negative, Gram-Positive Cocci from Blood Cultures by Partial 16S rRNA Gene Analysis and by Vitek 2 Examination.The open microbiology journal · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonhemolytic streptococci (NHS) cause serious infections, such as endocarditis and septicemia. Many conventional phenotypic methods are insufficient for the identification of bacteria in this group to the species level. Genetic analysis has revealed that single-gene analysis is insufficient for the identification of all species in this group of bacteria. The aim of the present study was to establish a method based on sequence analysis of the 16S-23S intergenic spacer (ITS) region and the partial gdh gene to identify clinical relevant NHS to the species level. Sequence analysis of the ITS region was performed with 57 NHS reference or clinical strains. Satisfactory identification to the species level was achieved for 14/19 NHS species included in this study on the basis of sequence analysis of the ITS region. Streptococcus salivarius and Streptococcus vestibularis obtained the expected taxon as the best taxon match, but there was a short maximum score distance to the next best match (distance, <10). Streptococcus mitis, Streptococcus oralis, and Streptococcus pneumoniae could not be unambiguously discriminated by sequence analysis of the ITS region, as was also proven by phylogenetic analysis. These five species could be identified to the group level only by ITS sequence analysis. Partial gdh sequence analysis was applied to the 11 S. oralis strains, the 11 S. mitis strains, and the 17 S. pneumoniae strains. All except one strain achieved a satisfactory identification to the species level. A phylogenetic algorithm based on the analysis of partial gdh gene sequences revealed three distinct clusters. We suggest that sequence analysis of the combination of the ITS region and the partial gdh gene can be used in the reference laboratory for the species-level identification of NHS.
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