ArticleJournal of clinical microbiology2009
Single-point mutations causing more than 100-fold underestimation of human immunodeficiency virus type 1 (HIV-1) load with the Cobas TaqMan HIV-1 real-time PCR assay.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Analysis of the Frequency of Mutations at Diagnostic Oligonucleotide Sites and Their Impact on the Efficiency of PCR for HIV-1.Microorganisms · 2023Article
- SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
- Nucleic acid testing and molecular characterization of HIV infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Diagnosis of Human Immunodeficiency Virus Infection.Clinical microbiology reviews · 2019Review
- Nucleoside analogs to manage sequence divergence in nucleic acid amplification and SNP detection.Nucleic acids research · 2018Article
- Real-time PCR assays for hepatitis B virus DNA quantification may require two different targets.Virology journal · 2017Article
- Evaluation of the Procleix Ultrio Elite Assay and the Panther-System for Individual NAT Screening of Blood, Hematopoietic Stem Cell, Tissue and Organ Donors.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2016Article
- Comparison of the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 test v1.0 with v2.0 in HIV-1 viral load quantification.The Kaohsiung journal of medical sciences · 2015Article
- A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting.Biological research · 2014Article
- Use of reverse-transcriptase-based HIV-1 viral load assessment to confirm low viral loads in newly diagnosed patients in Switzerland.BMC infectious diseases · 2014Article
- Risk Minimization Measures for Blood Screening HIV-1 Nucleic Acid Amplification Technique Assays in Germany.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2014Article
- A broadly reactive one-step SYBR Green I real-time RT-PCR assay for rapid detection of murine norovirus.PloS one · 2014Article
- Development, validation and clinical evaluation of a low cost in-house HIV-1 drug resistance genotyping assay for Indian patients.PloS one · 2014Article
- Cell-associated HIV RNA: a dynamic biomarker of viral persistence.Retrovirology · 2013Review
- Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens.Journal of clinical microbiology · 2013Article
- Article
- A sensitive branched DNA HIV-1 signal amplification viral load assay with single day turnaround.PloS one · 2012Article
- Molecular methods and platforms for infectious diseases testing a review of FDA-approved and cleared assays.The Journal of molecular diagnostics : JMD · 2011Review
- Evaluation of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test and identification of rare polymorphisms potentially affecting assay performance.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.
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Registered trials
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