ArticleACS chemical biology2009
Indo-1 derivatives for local calcium sensing.
Article in ACS chemical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag Protein.Bioconjugate chemistry · 2025Article
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- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
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- Bioorthogonal, Fluorogenic Targeting of Voltage-Sensitive Fluorophores for Visualizing Membrane Potential Dynamics in Cellular Organelles.Journal of the American Chemical Society · 2022Article
- Fluorescent and Bioluminescent Calcium Indicators with Tuneable Colors and Affinities.Journal of the American Chemical Society · 2022Article
- Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids.Chemical reviews · 2022Review
- Origins of CaBiochemistry · 2021Article
- Loss of dysferlin or myoferlin results in differential defects in excitation-contraction coupling in mouse skeletal muscle.Scientific reports · 2021Article
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling.BMC biology · 2019Article
- Visualization of long-term MgChemical science · 2017Article
- Exploring cells with targeted biosensors.The Journal of general physiology · 2017Review
- Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors.Analytical chemistry · 2016Article
- Genetically anchored fluorescent probes for subcellular specific imaging of hydrogen sulfide.The Analyst · 2016Article
- Focal calcium monitoring with targeted nanosensors at the cytosolic side of endoplasmic reticulum.Science and technology of advanced materials · 2016Article
- Synthesis of an azido-tagged low affinity ratiometric calcium sensor.Tetrahedron · 2015Article
- Advances in chemical labeling of proteins in living cells.Cell and tissue research · 2015Review
- Genetic targeting of chemical indicators in vivo.Nature methods · 2015Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The role of calcium in signal transduction relies on the precise spatial and temporal control of its concentration. The existing means to detect fluctuations in Ca2+ concentrations with adequate temporal and spatial resolution are limited. We introduce here a method to measure Ca2+ concentrations in defined locations in living cells that is based on linking the Ca2+-sensitive dye Indo-1 to SNAP-tag fusion proteins. Fluorescence spectroscopy of SNAP-Indo-1 conjugates in vitro showed that the conjugates retained the Ca2+-sensing ability of Indo-1. In a proof-of-principle experiment, local Ca2+ sensing was demonstrated in single cells dissociated from muscle of adult mice expressing a nucleus-localized SNAP-tag fusion. Ca2+ concentrations inside nuclei of resting cells were measured by shifted excitation and emission ratioing of confocal microscopic images of fluorescence. After permeabilizing the plasma membrane, changes in the bathing solution induced corresponding changes in nuclear [Ca2+] that were readily detected and used for a preliminary calibration of the technique. This work thus demonstrates the synthesis and application of SNAP-tag-based Ca2+ indicators that combine the spatial specificity of genetically encoded calcium indicators with the advantageous spectroscopic properties of synthetic indicators.
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