ArticleAnalytical chemistry2009
Fiber-optic microsphere-based antibody array for the analysis of inflammatory cytokines in saliva.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- Role of Salivary Biomarkers in Cystic Fibrosis: A Systematic Review.BioMed research international · 2022Pooled it
- An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.Lab on a chip · 2014Trial
- Integrated microfluidic platforms for extracellular vesicles: Separation, detection, and clinical translation.APL bioengineering · 2025Review
- Functionalized optical fiber ball-shaped biosensor for label-free, low-limit detection of IL-8 protein.Biomedical optics express · 2024Article
- Advances in the analysis of single extracellular vesicles: A critical review.Sensors and actuators reports · 2021Article
- Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance.Sensors (Basel, Switzerland) · 2021Review
- Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids.Sensors (Basel, Switzerland) · 2020Review
- Advancing Point-of-Care (PoC) Testing Using Human Saliva as Liquid Biopsy.Diagnostics (Basel, Switzerland) · 2017Review
- Saliva diagnostics - Current views and directions.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement.Sensors (Basel, Switzerland) · 2017Review
- Fiber-optic array using molecularly imprinted microspheres for antibiotic analysis.Chemical science · 2015Article
- Salivary diagnostics using a portable point-of-service platform: a review.Clinical therapeutics · 2015Review
- Bioanalytical chemistry of cytokines--a review.Analytica chimica acta · 2015Review
- Correlations of salivary biomarkers with clinical assessments in patients with cystic fibrosis.PloS one · 2015Article
- Salivary inflammatory mediator profiling and correlation to clinical disease markers in asthma.PloS one · 2014Article
- Multiplexed fluorescent microarray for human salivary protein analysis using polymer microspheres and fiber-optic bundles.Journal of visualized experiments : JoVE · 2013Article
- Which biomarkers are effective for identifying Th2-driven inflammation in asthma?Current allergy and asthma reports · 2013Review
- Multiplexed salivary protein profiling for patients with respiratory diseases using fiber-optic bundles and fluorescent antibody-based microarrays.Analytical chemistry · 2013Article
- Article
- Miniaturized technology for protein and nucleic acid point-of-care testing.Translational research : the journal of laboratory and clinical medicine · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Antibody microarrays have emerged as useful tools for high-throughput protein analysis and candidate biomarker screening. We describe here the development of a multiplexed microsphere-based antibody array capable of simultaneously measuring 10 inflammatory protein mediators. Cytokine-capture microspheres were fabricated by covalently coupling monoclonal antibodies specific for cytokines of interest to fluorescently encoded 3.1 microm polymer microspheres. An optical fiber bundle containing approximately 50,000 individual 3.1 microm diameter fibers was chemically etched to create microwells in which cytokine-capture microspheres could be deposited. Microspheres were randomly distributed in the wells to produce an antibody array for performing a multiplexed sandwich immunoassay. The array responded specifically to recombinant cytokine solutions in a concentration-dependent fashion. The array was also used to examine endogenous mediator patterns in saliva supernatants from patients with pulmonary inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD). This array technology may prove useful as a laboratory-based platform for inflammatory disease research and diagnostics, and its small footprint could also enable integration into a microfluidic cassette for use in point-of-care testing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.