ArticlePLoS neglected tropical diseases2009
Functional analysis of the cathepsin-like cysteine protease genes in adult Brugia malayi using RNA interference.
Article in PLoS neglected tropical diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 63 citations in OpenAlex.
- Silencing Ditylenchus destructor cathepsin L-like cysteine protease has negative pleiotropic effect on nematode ontogenesis.Scientific reports · 2024Article
- Discovery of New Broad-Spectrum Anti-Infectives for Eukaryotic Pathogens Using Bioorganometallic Chemistry.Journal of medicinal chemistry · 2023Article
- Molecular characterization of a novel serine proteinase from Trichinella spiralis and its participation in larval invasion of gut epithelium.PLoS neglected tropical diseases · 2023Article
- Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.Scientific reports · 2023Article
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- Localization and RNA Interference-Driven Inhibition of a Brugia malayi-Encoded Interleukin-5 Receptor Binding Protein.Infection and immunity · 2022Article
- Article
- Short-course quinazoline drug treatments are effective in the Litomosoides sigmodontis and Brugia pahangi jird models.International journal for parasitology. Drugs and drug resistance · 2020Article
- Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference.Parasites & vectors · 2018Article
- Cysteine proteases during larval migration and development of helminths in their final host.PLoS neglected tropical diseases · 2018Review
- Transcriptome-wide responses of adult melon thrips (Thrips palmi) associated with capsicum chlorosis virus infection.PloS one · 2018Article
- Functional genomics inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae.Parasites & vectors · 2017Article
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- Molecular characterization of a cathepsin F-like protease in Trichinella spiralis.Parasites & vectors · 2015Article
- A novel member of the let-7 microRNA family is associated with developmental transitions in filarial nematode parasites.BMC genomics · 2015Article
- Release of Small RNA-containing Exosome-like Vesicles from the Human Filarial Parasite Brugia malayi.PLoS neglected tropical diseases · 2015Article
- Cathepsin Gene Family Reveals Transcriptome Patterns Related to the Infective Stages of the Salmon Louse Caligus rogercresseyi.PloS one · 2015Article
- Potential involvement of Brugia malayi cysteine proteases in the maintenance of the endosymbiotic relationship with Wolbachia.International journal for parasitology. Drugs and drug resistance · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCathepsin-like enzymes have been identified as potential targets for drug or vaccine development in many parasites, as their functions appear to be essential in a variety of important biological processes within the host, such as molting, cuticle remodeling, embryogenesis, feeding and immune evasion. Functional analysis of Caenorhabditis elegans cathepsin L (Ce-cpl-1) and cathepsin Z (Ce-cpz-1) has established that both genes are required for early embryogenesis, with Ce-cpl-1 having a role in regulating in part the processing of yolk proteins. Ce-cpz-1 also has an important role during molting. METHODS AND
findingsRNA interference assays have allowed us to verify whether the functions of the orthologous filarial genes in Brugia malayi adult female worms are similar. Treatment of B. malayi adult female worms with Bm-cpl-1, Bm-cpl-5, which belong to group Ia of the filarial cpl gene family, or Bm-cpz-1 dsRNA resulted in decreased numbers of secreted microfilariae in vitro. In addition, analysis of the intrauterine progeny of the Bm-cpl-5 or Bm-cpl Pro dsRNA- and siRNA-treated worms revealed a clear disruption in the process of embryogenesis resulting in structural abnormalities in embryos and a varied differential development of embryonic stages.
conclusionsOur studies suggest that these filarial cathepsin-like cysteine proteases are likely to be functional orthologs of the C. elegans genes. This functional conservation may thus allow for a more thorough investigation of their distinct functions and their development as potential drug targets.
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