ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Inhibition of multidrug-resistant Acinetobacter baumannii by nonviral expression of hCAP-18 in a bioengineered human skin tissue.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Phase I/II clinical evaluation of StrataGraft: a consistent, pathogen-free human skin substitute.The Journal of trauma · 2009Trial
- Cathelicidins: Opportunities and Challenges in Skin Therapeutics and Clinical Translation.Antibiotics (Basel, Switzerland) · 2024Review
- Living Skin Substitute Tissue-Is a Replacement for the Autograft Possible?European burn journal · 2023Article
- Mammals' humoral immune proteins and peptides targeting the bacterial envelope: from natural protection to therapeutic applications against multidrug-resistant Gram-negatives.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Hydrophilic/hydrophobic characters of antimicrobial peptides derived from animals and their effects on multidrug resistant clinical isolates.Dong wu xue yan jiu = Zoological research · 2015Article
- Skin electroporation of a plasmid encoding hCAP-18/LL-37 host defense peptide promotes wound healing.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Topical antimicrobials for burn infections - an update.Recent patents on anti-infective drug discovery · 2013Review
- Biscatecholate-monohydroxamate mixed ligand siderophore-carbacephalosporin conjugates are selective sideromycin antibiotics that target Acinetobacter baumannii.Journal of medicinal chemistry · 2013Article
- Chimeric Human Skin Substitute Tissue: A Novel Treatment Option for the Delivery of Autologous Keratinocytes.Advances in wound care · 2012Article
- A Bioengineered Human Skin Tissue for the Treatment of Infected Wounds.Advances in wound care · 2012Article
- Clinical Evaluation of NIKS-Based Bioengineered Skin Substitute Tissue in Complex Skin Defects: Phase I/IIa Clinical Trial Results.Advances in wound care · 2012Article
- Chimeric composite skin substitutes for delivery of autologous keratinocytes to promote tissue regeneration.Annals of surgery · 2010Article
- Bioengineered human skin: working the bugs out.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: a therapeutic alternative for infected wounds.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyArticle
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12 authors at 2 institutions in 1 country.
Funding
Abstract
When skin is compromised, a cascade of signals initiates the rapid repair of the epidermis to prevent fluid loss and provide defense against invading microbes. During this response, keratinocytes produce host defense peptides (HDPs) that have antimicrobial activity against a diverse set of pathogens. Using nonviral vectors we have genetically modified the novel, nontumorigenic, pathogen-free human keratinocyte progenitor cell line (NIKS) to express the human cathelicidin HDP in a tissue-specific manner. NIKS skin tissue that expresses elevated levels of cathelicidin possesses key histological features of normal epidermis and displays enhanced antimicrobial activity against bacteria in vitro. Moreover, in an in vivo infected burn wound model, this tissue results in a two log reduction in a clinical isolate of multidrug-resistant Acinetobacter baumannii. Taken together, these results suggest that this genetically engineered human tissue could be applied to burns and ulcers to counteract bacterial contamination and prevent infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.