Evidence map›Paper›PMID 19190222›Full record

ArticleThe American journal of tropical medicine and hygiene2009

Genetic diversity of Trichomonas vaginalis clinical isolates determined by EcoRI restriction fragment length polymorphism of heat-shock protein 70 genes.

John C Meade, Jacqueline de Mestral, Jonathan K Stiles, W Evan Secor, Richard W Finley, John D Cleary, William B Lushbaugh

Open access · greenAbstract read
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.6field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. Microscopic and molecular detection ofFrontiers in microbiology · 2024
    Article
  3. Prevalence and Genotype ofJournal of tropical medicine · 2023
    Article
  4. Article
  5. Genotypic Variation inInfectious diseases in obstetrics and gynecology · 2020
    Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Genetic relatedness of Trichomonas vaginalis reference and clinical isolates.The American journal of tropical medicine and hygiene · 2010
    Article
  13. Article
  14. Article
  15. Considerable Genetic Diversity ofIranian journal of parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

John C MeadeDepartment of Microbiology, Division of Infectious Diseases, University of Mississippi Medical Center, Jackson, Mississippi 39216-4505, USA. jmeade@microbio.umsmed.edu
Jacqueline de Mestral
Jonathan K Stiles
W Evan Secor
Richard W Finley
John D Cleary
William B Lushbaugh
University of Mississippi Medical Center · US

Funding

Cerebral Malaria Neurological Disorders in IndiaR21TW006804 · FIC · MOREHOUSE SCHOOL OF MEDICINE · PI STILES, JONATHAN K. · 2003 to 2005
$355k
FIC NIH HHS R21 TW006804
6 · The paper itself

Abstract

Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with EcoRI-digested genomic DNA was used in molecular typing of 129 Trichomonas vaginalis isolates. Results indicate that Trichomonas organisms exhibit considerable polymorphism in their Hsp70 RFLP patterns. Analysis of seven American Type Culture Collection reference strains and 122 clinical isolates, including 84 isolates from Jackson, Mississippi, 18 isolates from Atlanta, Georgia, and 20 isolates from throughout the United States, showed 105 distinct Hsp70 RFLP pattern subtypes for Trichomonas. Phylogenetic analysis of the Hsp70 RFLP data showed that the T. vaginalis isolates were organized into two clonal lineages. These results illustrate the substantial genomic diversity present in T. vaginalis and indicate that a large number of genetically distinct Trichomonas isolates may be responsible for human trichomoniasis in the United States.

Indexed as

Genetic VariationAnimalsBacterial Typing TechniquesDeoxyribonuclease EcoRIFemaleHSP70 Heat-Shock ProteinsHumansMalePhylogenyPolymorphism, Restriction Fragment LengthTrichomonas vaginalisTrichomonas VaginitisDeoxyribonuclease EcoRIHSP70 Heat-Shock Proteins

Identifiers

PMID19190222
PMCPMC2786270
OpenAlexW2112404509

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.