ArticleClinical cancer research : an official journal of the American Association for Cancer Research2009
Distinct BRAF (V600E) and KRAS mutations in high microsatellite instability sporadic colorectal cancer in African Americans.
Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 2 syntheses or guidelines pooled it, 73 citations in OpenAlex.
- A meta-analysis of MSI frequency and race in colorectal cancer.Oncotarget · 2016Pooled it
- MLH1 promoter methylation frequency in colorectal cancer patients and related clinicopathological and molecular features.PloS one · 2013Pooled it
- Article
- Predictive Biomarkers and Resistance Mechanisms of Checkpoint Inhibitors in Malignant Solid Tumors.International journal of molecular sciences · 2024Review
- Molecular and Sociodemographic Colorectal Cancer Disparities in Latinos Living in Puerto Rico.Genes · 2023Article
- Clinicopathological characteristics of high microsatellite instability/mismatch repair-deficient colorectal cancer: A narrative review.Frontiers in immunology · 2022Review
- Disparities in Surgical Oncology: Management of Advanced Cancer.Annals of surgical oncology · 2021Review
- Determination of distinctive hypomethylated genes in African American colorectal neoplastic lesions.Therapeutic advances in gastroenterology · 2020Article
- Screening of MSI detection loci and their heterogeneity in East Asian colorectal cancer patients.Cancer medicine · 2019Article
- Downregulated Tim-3 expression is responsible for the incidence and development of colorectal cancer.Oncology letters · 2018Article
- Article
- Targeted exome sequencing reveals distinct pathogenic variants in Iranians with colorectal cancer.Oncotarget · 2017Article
- Article
- Patterns of Sociodemographic and Clinicopathologic Characteristics of Stages II and III Colorectal Cancer Patients by Age: Examining Potential Mechanisms of Young-Onset Disease.Journal of cancer epidemiology · 2017Article
- Genomics of Colorectal Cancer in African Americans.Next generation, sequencing & applications · 2016Article
- Prognostic impact of mutation profiling in patients with stage II and III colon cancer.Scientific reports · 2016Article
- Promoter methylation of ITF2, but not APC, is associated with microsatellite instability in two populations of colorectal cancer patients.BMC cancer · 2016Article
- Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans.Gastroenterology research and practice · 2016Article
- Racial Differences in BRAF/KRAS Mutation Rates and Survival in Stage III Colon Cancer Patients.Journal of the National Cancer Institute · 2015Article
- Epigenetics could explain some Moroccan population colorectal cancers peculiarities: microsatellite instability pathway exploration.Diagnostic pathology · 2015Observational
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeColorectal cancer develops through genetic, epigenetic, and environmental events that result in uncontrolled cell proliferation. Colorectal cancer incidence and mortality is higher in African Americans (AA) than in the general population. Here, we carried out a molecular analysis of sporadic colorectal cancer tumors from AAs to investigate possible explanations for the observed disparities. EXPERIMENTAL
designA total of 222 AA colorectal cancer tumors were analyzed for microsatellite instability (MSI) for protein expression of two DNA mismatch repair genes, MLH1 and MSH2, by immunohistochemistry; for the methylation silencing of MLH1, p16, APC, and APC2 promoters by methylation-specific PCR; and for point mutations in two oncogenes, KRAS and BRAF, by sequencing.
resultsIn our sample, 19.8% of the AAs colorectal cancer tumors were MSI high (MSI-H) and did not associate with any of the clinicopathologic features, except tumor differentiation. Higher levels of inactive DNA mismatch repair proteins MLH1 (41%) and MSH2 (33%) were found by immunohistochemistry. Methylation-specific PCR analysis revealed a high level of methylation for MLH1 (66%), APC (53%), and APC2 (90%), but not for p16 (26%). BRAF mutations were only within the MSI-H tumors, whereas most (64%) of KRAS mutations were found within the non-MSI-H group.
conclusionsMLH1, MSH2, and BRAF alterations are significantly associated with MSI-H phenotype, unlike APC, APC2 and KRAS alterations. The prominent role of DNA mismatch repair gene suppression in MSI-H and a distinctive role of BRAF and KRAS mutations with respect to MSI status are supported by this study.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.