ArticleNucleic acids research2009
A minimized rRNA-binding site for ribosomal protein S4 and its implications for 30S assembly.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Enhancement of heat resistance ofEngineering microbiology · 2026Article
- Fibrillarin/Nop1 perturbs RNA folding and assembly independently of liquid-liquid phase separation.Nucleic acids research · 2026Article
- Disassembly of unstable RNA structures by an E. coli DEAD-box chaperone accelerates ribosome assembly.Nucleic acids research · 2025Article
- Ribosomal Protein S12 Hastens Nucleation of Co-Transcriptional Ribosome Assembly.Biomolecules · 2023Article
- Pseudouridine Synthase RsuA Captures an Assembly Intermediate that Is Stabilized by Ribosomal Protein S17.Biomolecules · 2020Article
- RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.Nucleic acids research · 2020Article
- Article
- Size-variable zone in V3 region of 16S rRNA.RNA biology · 2017Article
- Modular Organization of Residue-Level Contacts Shapes the Selection Pressure on Individual Amino Acid Sites of Ribosomal Proteins.Genome biology and evolution · 2017Article
- Differential effects of ribosomal proteins and Mg2+ ions on a conformational switch during 30S ribosome 5'-domain assembly.RNA (New York, N.Y.) · 2015Article
- Article
- Specific contacts between protein S4 and ribosomal RNA are required at multiple stages of ribosome assembly.RNA (New York, N.Y.) · 2013Article
- Assembly of the five-way junction in the ribosomal small subunit using hybrid MD-Gō simulations.The journal of physical chemistry. B · 2012Article
- Slow formation of stable complexes during coincubation of minimal rRNA and ribosomal protein S4.Journal of molecular biology · 2011Article
- Functional role of ribosomal signatures.Biophysical journal · 2010Article
- Global stabilization of rRNA structure by ribosomal proteins S4, S17, and S20.Journal of molecular biology · 2009Article
- Analysis of RNA folding by native polyacrylamide gel electrophoresis.Methods in enzymology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Primary ribosomal protein S4 is essential for 30S ribosome biogenesis in eubacteria, because it nucleates subunit assembly and helps coordinate assembly with the synthesis of its rRNA and protein components. S4 binds a five-helix junction (5WJ) that bridges the 5' and 3' ends of the 16S 5' domain. To delineate which nucleotides contribute to S4 recognition, sequential deletions of the 16S 5' domain were tested in competitive S4-binding assays based on electrophoretic mobility shifts. S4 binds the minimal 5WJ RNA containing just the five-helix junction as well or better than with affinity comparable to or better than the 5' domain or native 16S rRNA. Internal deletions and point mutations demonstrated that helices 3, 4, 16 and residues at the helix junctions are necessary for S4 binding, while the conserved helix 18 pseudoknot is dispensable. Hydroxyl radical footprinting and chemical base modification showed that S4 makes the same interactions with minimal rRNA substrates as with the native 16S rRNA, but the minimal substrates are more pre-organized for binding S4. Together, these results suggest that favorable interactions with S4 offset the energetic penalty for folding the 16S rRNA.
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