ArticleEuropean biophysics journal : EBJ2009
Insights into protein-polysorbate interactions analysed by means of isothermal titration and differential scanning calorimetry.
Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 79 citations in OpenAlex.
- Pharmaceutical Analysis of Protein-Peptide Coformulations and the Influence of Polysorbates.Molecular pharmaceutics · 2025Article
- A Mechanistic Understanding of Monoclonal Antibody Interfacial Protection by Hydrolytically Degraded Polysorbate 20 and 80 under IV Bag Conditions.Pharmaceutical research · 2022Article
- Adsorption of non-ionic surfactant and monoclonal antibody on siliconized surface studied by neutron reflectometry.Journal of colloid and interface science · 2021Article
- Protein Loading into Spongelike PLGA Microspheres.Pharmaceutics · 2021Article
- Albumin displacement at the air-water interface by Tween (Polysorbate) surfactants.European biophysics journal : EBJ · 2020Article
- Anaphylaxis to three humanized antibodies for severe asthma: a case study.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2020Article
- Effect of Polysorbate 20 and Polysorbate 80 on the Higher-Order Structure of a Monoclonal Antibody and Its Fab and Fc Fragments Probed Using 2D Nuclear Magnetic Resonance Spectroscopy.Journal of pharmaceutical sciences · 2017Article
- The role of select subtype polymorphisms on HIV-1 protease conformational sampling and dynamics.The Journal of biological chemistry · 2014Article
- Stability of lysozyme in aqueous extremolyte solutions during heat shock and accelerated thermal conditions.PloS one · 2014Article
- Disruption of Escherichia coli amyloid-integrated biofilm formation at the air-liquid interface by a polysorbate surfactant.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Biophysical mechanisms of the neutralization of endotoxins by lipopolyamines.The open biochemistry journal · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Therapeutic proteins formulated as liquid solutions at high protein concentration are very sensitive to chemical and physical degradation. Especially avoiding the formation of protein aggregates is very crucial for product quality. In order to stabilize the colloidal properties of protein therapeutics various excipient are used. Especially the detergents polysorbate 20 and 80 are common. However, the mechanism upon which the detergents protect the protein from aggregation is not really known. The present study investigates the interaction of polysorbate 20 and 80 with different proteins: lysozyme, bovine serum albumin (BSA) and an immunoglobulin. The interaction and binding of the detergents to the proteins is investigated by isothermal titration calorimetry (ITC). From ITC the thermodynamic parameters (DeltaH: change in enthalpy, DeltaS: entropy and DeltaG: free energy) upon binding are derived as well as the binding constant K (a). The thermal stability of the proteins in the presence of the detergent is assessed by differential scanning calorimetry (DSC). The results show that both detergents bind to BSA with K (a) between 8 and 12 x 10(3) M(-1) with DeltaH -50 to -60 kJ/mol (25 degrees C). One to two detergent molecules bind to BSA. The presence of both detergents induces a weak stabilisation of the thermal denaturation properties of BSA. However, the interaction of polysorbate 20 and 80 with lysozyme and the immunoglobulin is quite negligible. The presence of the detergents up to a concentration of 2 mM has no impact on the heat capacity curve neither a destabilisation nor a stabilisation of the native conformation is observed.
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