ArticleBlood2009
Forward RNAi screens in primary human hematopoietic stem/progenitor cells.
Article in Blood, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- NDR1 increases NOTCH1 signaling activity by impairing Fbw7 mediated NICD degradation to enhance breast cancer stem cell properties.Molecular medicine (Cambridge, Mass.) · 2022Article
- Sister chromatid cohesion defects are associated with chromosomal copy number heterogeneity in high hyperdiploid childhood acute lymphoblastic leukemia.Genes, chromosomes & cancer · 2021Article
- Combined lentiviral- and RNA-mediated CRISPR/Cas9 delivery for efficient and traceable gene editing in human hematopoietic stem and progenitor cells.Scientific reports · 2020Article
- Comparative RNAi Screens in Isogenic Human Stem Cells Reveal SMARCA4 as a Differential Regulator.Stem cell reports · 2019Article
- Transient inhibition of NF-κB signaling enhancesHaematologica · 2018Article
- Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells.Blood · 2017Article
- Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling.The Journal of biological chemistry · 2016Article
- Genome-wide RNAi Screen Identifies Cohesin Genes as Modifiers of Renewal and Differentiation in Human HSCs.Cell reports · 2016Article
- Functional screen identifies regulators of murine hematopoietic stem cell repopulation.The Journal of experimental medicine · 2016Article
- Jarid2 regulates hematopoietic stem cell function by acting with polycomb repressive complex 2.Blood · 2015Article
- Genome-wide RNAi screening identifies genes inhibiting the migration of glioblastoma cells.PloS one · 2013Article
- An in vivo functional screen uncovers miR-150-mediated regulation of hematopoietic injury response.Cell reports · 2012Article
- Deeply dissecting stemness: making sense to non-coding RNAs in stem cells.Stem cell reviews and reports · 2012Review
- Allogeneic haematopoietic stem cell transplantation: individualized stem cell and immune therapy of cancer.Nature reviews. Cancer · 2010Review
- Genomic screening with RNAi: results and challenges.Annual review of biochemistry · 2010Review
- Functional genomic screening approaches in mechanistic toxicology and potential future applications of CRISPR-Cas9.Mutation research. Reviews in mutation researchReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
Abstract
The mechanisms regulating key fate decisions such as self-renewal and differentiation in hematopoietic stem and progenitor cells (HSPC) remain poorly understood. We report here a screening strategy developed to assess modulators of human hematopoiesis using a lentiviral short hairpin RNA (shRNA) library transduced into cord blood-derived stem/progenitor cells. To screen for modifiers of self-renewal/differentiation, we used the limited persistence of HSPCs under ex vivo culture conditions as a baseline for functional selection of shRNAs conferring enhanced maintenance or expansion of the stem/progenitor potential. This approach enables complex, pooled screens in large numbers of cells. Functional selection identified novel specific gene targets (exostoses 1) or shRNA constructs capable of altering human hematopoietic progenitor differentiation or stem cell expansion, respectively, thereby demonstrating the potential of this forward screening approach in primary human stem cell populations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.