ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2009
In situ zymography and immunolabeling in fixed and decalcified craniofacial tissues.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- In Situ Zymography in Formalin-Fixed Paraffin-Embedded and Mineralized Tissues.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Gelatin Zymography Can Be Performed on Fixed Brain Tissue.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Article
- Collagenase-Cleavable Peptide Amphiphile Micelles as a Novel Theranostic Strategy in Atherosclerosis.Advanced therapeutics · 2020Article
- Histological, histochemical, and protein changes after induced malocclusion by occlusion alteration of Wistar rats.BioMed research international · 2014Article
- Senescent fibroblasts enhance early skin carcinogenic events via a paracrine MMP-PAR-1 axis.PloS one · 2013Article
- Detection of gelatinolytic activity in developing basement membranes of the mouse embryo head by combining sensitive in situ zymography with immunolabeling.Histochemistry and cell biology · 2012Article
- Lens placode modulates extracellular matrix formation during early eye development.Differentiation; research in biological diversityArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In situ zymography is a very important technique that shows the proteolytic activity in sections and allows researchers to observe the specific sites of proteolysis in tissues or cells. It is normally performed in non-fixed frozen sections and is not routinely performed in calcified tissues. In this study, we describe a technique that maintains proteolytic activity in fixed and decalcified sections obtained after routine paraffin sectioning in conventional microtome and cryostat sections. We used adult rat hemimandibles, which presented bone, enamel, and dentine matrices; the substrate used was dye-quenched-gelatin. Gelatinolytic activity was colocalized with MMP-2 using fluorescent antibodies. Specific proteolytic activity was observed in all sections, compatible with metalloproteinase activity, particularly in dentine and bone. Furthermore, matrix metalloproteinase-2 was colocalized to the sites of green fluorescence in dentine. In conclusion, the technique presented here will allow in situ zymography reactions in fixed, decalcified, and paraffin-embedded tissues, and we showed that paraformaldehyde-lysine-periodate-fixed cryostat sections are suitable for colocalization of gelatinolytic activity and protein labeling with antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.