ArticleThe Journal of biological chemistry2009
Identification of myo-inositol-3-phosphate synthase isoforms: characterization, expression, and putative role of a 16-kDa gamma(c) isoform.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Myo-inositol rescued insulin resistance and dyslipidemia in db/db mice.Journal of applied biomedicine · 2024Article
- Myo-Inositol in Fermented Sugar Matrix Improves Human Macrophage Function.Molecular nutrition & food research · 2022Article
- Inositols Depletion and Resistance: Principal Mechanisms and Therapeutic Strategies.International journal of molecular sciences · 2021Review
- Musashi2 promotes the progression of pancreatic cancer through a novel ISYNA1-p21/ZEB-1 pathway.Journal of cellular and molecular medicine · 2020Article
- Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling.Scientific reports · 2020Article
- Review
- Myo-inositol concentration in MR spectroscopy for differentiating high grade glioma from primary central nervous system lymphoma.Journal of neuro-oncology · 2018Article
- Nutritional and Acquired Deficiencies in Inositol Bioavailability. Correlations with Metabolic Disorders.International journal of molecular sciences · 2017Review
- Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus): effect of seawater acclimation.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Inositol Hexakisphosphate Kinase 1 (IP6K1) Regulates Inositol Synthesis in Mammalian Cells.The Journal of biological chemistry · 2016Article
- Inositol synthesis regulates the activation of GSK-3α in neuronal cells.Journal of neurochemistry · 2015Article
- Direct Ionic Regulation of the Activity of Myo-Inositol Biosynthesis Enzymes in Mozambique Tilapia.PloS one · 2015Article
- Biosynthesis of a central intermediate in hydrogen sulfide metabolism by a novel human sulfurtransferase and its yeast ortholog.Biochemistry · 2014Article
- Derivation and osmotolerance characterization of three immortalized tilapia (Oreochromis mossambicus) cell lines.PloS one · 2014Article
- Salinity-induced regulation of the myo-inositol biosynthesis pathway in tilapia gill epithelium.The Journal of experimental biology · 2013Article
- Tilapia (Oreochromis mossambicus) brain cells respond to hyperosmotic challenge by inducing myo-inositol biosynthesis.The Journal of experimental biology · 2013Article
- Phosphorylation regulates myo-inositol-3-phosphate synthase: a novel regulatory mechanism of inositol biosynthesis.The Journal of biological chemistry · 2013Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Rat brain myo-inositol 3-phosphate synthase is a phosphoprotein.Molecular and cellular biochemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Myo-inositol is an important constituent of membrane phospholipids and is a precursor for the phosphoinositide signaling pathway. It is synthesized from glucose 6-phosphate by myo-inositol-3-phosphate synthase (IP synthase), a homotrimer composed of a 68-kDa polypeptide in most mammalian tissues. It is a putative target for mood-stabilizing drugs such as lithium and valproate. Here, we show that the rat gene (Isyna1) encoding this enzyme generates a number of alternatively spliced transcripts in addition to the fully spliced form that encodes the 68-kDa subunit (the alpha isoform). Specifically, we identify a small 16-kDa subunit (the gamma(c) isoform) derived by an intron retention mechanism and provide evidence for its existence in rat tissues. The gamma(c) isoform is highly conserved in mammals, but it lacks the catalytic domain while retaining the NAD(+) binding domain. Both alpha and gamma(c) isoforms are predominantly expressed in many rat tissues and display apparent stoichiometry in purified enzyme preparations. An IP synthase polyclonal antibody not only detects the alpha and gamma(c) isoforms but also several other isoforms in pancreas, intestine, and testis suggesting that the holoenzyme is composed of unique subunits in various tissues. Interestingly, the alpha isoform is not expressed in the intestine. IP synthase activity assays using purified alpha and gamma(c) isoforms indicate that the latter negatively modulates alpha isoform activity, possibly by competing for NAD(+) molecules. Our findings have important ramifications for understanding the mood stabilization process and suggest that inositol biosynthesis is a highly regulated and dynamic process.
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