ArticleMolecular cell2009
Enhancement of the seed-target recognition step in RNA silencing by a PIWI/MID domain protein.
Article in Molecular cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
87 citing papers in PubMed, 160 citations in OpenAlex.
- PIWI-Interacting RNAs in Cardiovascular Disease: From Epigenetic Regulators to Clinical Biomarkers and Therapeutic Targets.Reviews in cardiovascular medicine · 2026Review
- Functional microRNA targeting without seed pairing.Nucleic acids research · 2025Article
- Structural and evolutionary determinants of Argonaute function.Nucleic acids research · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.Molecular cell · 2024Article
- RNA-guided RNA silencing by an Asgard archaeal Argonaute.Nature communications · 2024Article
- The guide RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.bioRxiv : the preprint server for biology · 2024Article
- MnChemical science · 2024Article
- The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.Nucleic acids research · 2024Article
- Determining the defining lengths between mature microRNAs/small interfering RNAs and tinyRNAs.Scientific reports · 2023Article
- Determining the defining lengths between mature microRNAs/small interfering RNAs and tinyRNAs.bioRxiv : the preprint server for biology · 2023Article
- Insights in piRNA targeting rules.Wiley interdisciplinary reviews. RNA · 2023Article
- A tiny loop in the Argonaute PIWI domain tunes small RNA seed strength.EMBO reports · 2023Article
- Structural basis for sequence-specific recognition of guide and target strands by the Archaeoglobus fulgidus Argonaute protein.Scientific reports · 2023Article
- Article
- High-throughput biochemical profiling reveals functional adaptation of a bacterial Argonaute.Molecular cell · 2022Article
- 3D Modeling of Non-coding RNA Interactions.Advances in experimental medicine and biology · 2022Article
- Prokaryotic Argonaute Proteins as a Tool for Biotechnology.Molecular biology · 2022Article
- Characterization of Argonaute nucleases from mesophilic bacteria Paenibacillus borealis and Brevibacillus laterosporus.Bioresources and bioprocessing · 2021Article
- PiRNA pathway in the cardiovascular system: a novel regulator of cardiac differentiation, repair and regeneration.Journal of molecular medicine (Berlin, Germany) · 2021Review
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Target recognition in RNA silencing is governed by the "seed sequence" of a guide RNA strand associated with the PIWI/MID domain of an Argonaute protein in RISC. Using a reconstituted in vitro target recognition system, we show that a model PIWI/MID domain protein confers position-dependent tightening and loosening of guide-strand-target interactions. Over the seed sequence, the interaction affinity is enhanced up to approximately 300-fold. Enhancement is achieved through a reduced entropy penalty for the interaction. In contrast, interactions 3' of the seed are inhibited. We quantified mismatched target recognition inside and outside the seed, revealing amplified discrimination at the third position in the seed mediated by the PIWI/MID domain. Thus, association of the guide strand with the PIWI/MID domain generates an enhanced affinity anchor site over the seed that can promote fidelity in target recognition and stabilize and guide the assembly of the active silencing complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.