ArticleTropical medicine & international health : TM & IH2009
A new approach to characterize populations of Schistosoma mansoni from humans: development and assessment of microsatellite analysis of pooled miracidia.
Article in Tropical medicine & international health : TM & IH, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Genotypic-specific heat shock response of vector susceptibility toEcosphere (Washington, D.C) · 2022Article
- Population Structure and Dynamics of Helminthic Infection: Schistosomiasis.Microbiology spectrum · 2019Review
- The effect of sample size on estimates of genetic differentiation and effective population size for Schistosoma mansoni populations.International journal for parasitology · 2018Article
- Host immunity, nutrition and coinfection alter longitudinal infection patterns of schistosomes in a free ranging African buffalo population.PLoS neglected tropical diseases · 2017Article
- Repeated praziquantel treatments remodel the genetic and spatial landscape of schistosomiasis risk and transmission.International journal for parasitology · 2016Article
- The relative contribution of immigration or local increase for persistence of urban schistosomiasis in Salvador, Bahia, Brazil.PLoS neglected tropical diseases · 2015Article
- No apparent reduction in schistosome burden or genetic diversity following four years of school-based mass drug administration in mwea, central kenya, a heavy transmission area.PLoS neglected tropical diseases · 2014Article
- Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts.Molecular and biochemical parasitology · 2013Article
- Characteristics of the human host have little influence on which local Schistosoma mansoni populations are acquired.PLoS neglected tropical diseases · 2013Article
- Non-invasive sampling of schistosomes from humans requires correcting for family structure.PLoS neglected tropical diseases · 2013Article
- Review: analysis of parasite and other skewed counts.Tropical medicine & international health : TM & IH · 2012Review
- Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil.International journal for parasitology · 2011Article
- Long-term genetic stability and population dynamics of laboratory strains of Schistosoma mansoni.The Journal of parasitology · 2010Article
- Applying evolutionary genetics to schistosome epidemiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2010Review
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Authors and funding
7 authors.
Funding
Abstract
objectivesTo develop and assess a microsatellite technique to characterize populations of Schistosoma mansoni from humans.
methodsFor each of five patients, we calculated the allele count and frequency at 11 loci for several pools of miracidia (50 and 100), and compared these to population values, determined by amplifying microsatellites from 186 to 200 individual miracidia per patient.
resultsWe were able to detect up to 94.5% of alleles in pools. Allele count and frequency strongly and significantly correlated between singles and pools; marginally significant differences (P < 0.05) were detected for one patient (pools of 50) for allele frequencies and for two patients (pools of 100) for allele counts. Kato-Katz egg counts and number of alleles per pool did not co-vary, indicating that further direct comparisons of the results from these two techniques are needed.
conclusionsAllele counts and frequency profiles from pooling provide important information about infection intensity and complexity, beyond that obtained from traditional methods. Although we are not advocating use of pooling to replace individual genotyping studies, it can potentially be useful in certain applications as a rapid and cost effective screening method for studies of S. mansoni population genetics, or as a more informative way to quantify and characterize human worm populations.
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