ArticleEnvironmental microbiology2009
Synergistic role of curli and cellulose in cell adherence and biofilm formation of attaching and effacing Escherichia coli and identification of Fis as a negative regulator of curli.
Article in Environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
117 citing papers in PubMed, 220 citations in OpenAlex.
- Wrinkle-like structures emerge from matrix complementarity and mechanical discontinuity in heterogenous biofilms.NPJ biofilms and microbiomes · 2026Article
- From Environmental Persistence to Host Adaptation: Differential Curli Regulation inMicroorganisms · 2026Review
- From Cryptic Clade to Emerging Pathogen: Exploring the Evolutionary Divergence and Clinical Relevance ofMicroorganisms · 2026Review
- Genomic Characteristics of a Multidrug-Resistant Extraintestinal PathogenicMicroorganisms · 2026Article
- Pathotype-specific antimicrobial resistance in diarrheagenicFrontiers in microbiology · 2026Review
- Enhancing sanitation efficiency in red meat processing: a novel enzymatic approach.Frontiers in microbiology · 2026Article
- The Nucleoid-Associated Protein Fis Represses Type 3 Fimbriae to Modulate Biofilm and Adherence Formation inMicroorganisms · 2025Article
- Serrapeptase EliminatesMicroorganisms · 2025Article
- RcsB and H-NS Both Contribute to the Repression the Expression of theMicroorganisms · 2025Article
- Deleting fis downregulates virulence and effectively protects Pasteurella multocida infection in mice.BMC veterinary research · 2025Article
- Physical communication pathways in bacteria: an extra layer to quorum sensing.Biophysical reviews · 2025Review
- Antibiofilm Activities of Halogenated Pyrimidines Against EnterohemorrhagicInternational journal of molecular sciences · 2025Article
- Newly identified c-di-GMP pathway putative EAL domain gene STM0343 regulates stress resistance and virulence in Salmonella enterica serovar Typhimurium.Veterinary research · 2025Article
- Fluoropyrimidines affectBiofilm · 2024Article
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- Motility mediates satellite formation in confined biofilms.The ISME journal · 2023Article
- CsgI (YccT) Is a Novel Inhibitor of Curli Fimbriae Formation inInternational journal of molecular sciences · 2023Article
- Bacterial biofilms in the human body: prevalence and impacts on health and disease.Frontiers in cellular and infection microbiology · 2023Review
- Clonal groups of extended-spectrum β-lactamase and biofilm producing uropathogenicPathogens and global health · 2022Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Curli are adhesive fimbriae of Escherichia coli and Salmonella enterica. Expression of curli (csgA) and cellulose (bcsA) is co-activated by the transcriptional activator CsgD. In this study, we investigated the contribution of curli and cellulose to the adhesive properties of enterohaemorragic (EHEC) O157:H7 and enteropathogenic E. coli (EPEC) O127:H6. While single mutations in csgA, csgD or bcsA in EPEC and EHEC had no dramatic effect on cell adherence, double csgAbcsA mutants were significantly less adherent than the single mutants or wild-type strains to human colonic HT-29 epithelial cells or to cow colon tissue in vitro. Overexpression of csgD (carried on plasmid pCP994) in a csgD mutant, but not in the single csgA or bscA mutants, led to significant increase in adherence and biofilm formation in EPEC and EHEC, suggesting that synchronized over-production of curli and cellulose enhances bacterial adherence. In line with this finding, csgD transcription was activated significantly in the presence of cultured epithelial cells as compared with growth in tissue culture medium. Analysis of the influence of virulence and global regulators in the production of curli in EPEC identified Fis (factor for inversion stimulation) as a, heretofore unrecognized, negative transcriptional regulator of csgA expression. An EPEC E2348/69Deltafis produced abundant amounts of curli whereas a double fis/csgD mutant yielded no detectable curli production. Our data suggest that curli and cellulose act in concert to favour host colonization, biofilm formation and survival in different environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.