ArticleJournal of applied microbiology2009
Analysis of dye binding by and membrane potential in spores of Bacillus species.
Article in Journal of applied microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A High-Throughput Screening Strategy forMicroorganisms · 2025Article
- Combination of fluorescent reagents with 2-(4-aminophenyl) benzothiazole and safranin O was useful for analysis of spore structure, indicating the diversity ofFrontiers in microbiology · 2025Article
- A new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereus.Scientific reports · 2023Article
- Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery.Microbial biotechnology · 2022Article
- Changes in the Spore Proteome ofMicroorganisms · 2022Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Bringing Microbiology to Light: Toward All-Optical Electrophysiology in Bacteria.Bioelectricity · 2021Article
- Visualization of Germination Proteins in PutativeInternational journal of molecular sciences · 2020Article
- Assessment of Gram- and Viability-Staining Methods for Quantifying Bacterial Community Dynamics Using Flow Cytometry.Frontiers in microbiology · 2020Article
- Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating.PloS one · 2019Article
- Analysis of killing of growing cells and dormant and germinated spores of Bacillus species by black silicon nanopillars.Scientific reports · 2017Article
- Schrödinger's microbes: Tools for distinguishing the living from the dead in microbial ecosystems.Microbiome · 2017Review
- Sporicidal activity of ceragenin CSA-13 against Bacillus subtilis.Scientific reports · 2017Article
- Changes in Bacillus Spore Small Molecules, rRNA, Germination, and Outgrowth after Extended Sublethal Exposure to Various Temperatures: Evidence that Protein Synthesis Is Not Essential for Spore Germination.Journal of bacteriology · 2016Article
- Bacterial imaging and photodynamic inactivation using zinc(II)-dipicolylamine BODIPY conjugates.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Article
- The spore differentiation pathway in the enteric pathogen Clostridium difficile.PLoS genetics · 2013Article
- Intracellular vesicles as reproduction elements in cell wall-deficient L-form bacteria.PloS one · 2012Article
- Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.Molecular microbiology · 2011Article
- Constructing fluorogenic Bacillus spores (F-spores) via hydrophobic decoration of coat proteins.PloS one · 2010Article
- Characterization of spores of Bacillus subtilis that lack most coat layers.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
aimsTo determine roles of coats in staining Bacillus subtilis spores, and whether spores have membrane potential. METHODS AND
resultsStaining by four dyes and autofluorescence of B. subtilis spores that lack some (cotE, gerE) or most (cotE gerE) coat protein was measured. Wild-type, cotE and gerE spores autofluorescenced and bound dyes, but cotE gerE spores did not autofluorescence and were stained only by two dyes. A membrane potential-sensitive dye DiOC6(3) bound to dormant Bacillus megaterium and B. subtilis spores. While this binding was abolished by the protonophore FCCP, DiOC6(3) bound to heat-killed spores, but not to dormant B. subtilis cotE gerE spores. However, DiOC6(3) bound well to all germinated spores.
conclusionsThe autofluorescence of dormant B. subtilis spores and the binding of some dyes are due to the coat. There is no membrane potential in dormant Bacillus spores, although membrane potential is generated when spores germinate. SIGNIFICANCE AND IMPACT OF THE STUDY: The elimination of the autofluorescence of B. subtilis spores may allow assessment of the location of low abundance spore proteins using fluorescent reporter technology. The dormant spore's lack of membrane potential may allow tests of spore viability by assessing membrane potential in germinating spores.
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