ArticleBiophysical journal2009
Versatile horizontal force probe for mechanical tests on pipette-held cells, particles, and membrane capsules.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- Micropipette-based biomechanical nanotools on living cells.European biophysics journal : EBJ · 2022Review
- Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies.Scientific reports · 2018Article
- Micropipette force probe to quantify single-cell force generation: application to T-cell activation.Molecular biology of the cell · 2017Article
- Single lipid extraction: the anchoring strength of cholesterol in liquid-ordered and liquid-disordered phases.Biophysical journal · 2014Article
- Salivary proteins as predictors and controls for oral health.Journal of cell communication and signaling · 2011Article
- Adhesion frequency assay for in situ kinetics analysis of cross-junctional molecular interactions at the cell-cell interface.Journal of visualized experiments : JoVE · 2011Article
- Single-cell adhesion tests against functionalized microspheres arrayed on AFM cantilevers confirm heterophilic E- and N-cadherin binding.Biophysical journal · 2010Article
- Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells.Nature methods · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We present a multipurpose nanomechanical force probe that combines a sideways-mounted elastic cantilever and an optical-lever detection module with automated micropipette manipulation. It allows us to apply and measure compression, stretching, adhesion, and dissociation forces in the horizontal direction while providing a "side view" of ongoing experiments. The integrated micropipette setup facilitates the easy manipulation and mechanical interrogation of individual cells, functionalized particles, and synthetic membrane capsules. Pipette-held test objects are translated perpendicularly to and from the stationary cantilever, eliminating the need to attach them to a carrier surface and substantially reducing unwanted hydrodynamic coupling effects. Moreover, the test objects can be brought into contact with the cantilever anywhere along its length, which considerably enlarges the range of forces that can be applied with a single cantilever. Advantages of this instrument are demonstrated in example measurements of single-cell compression, membrane-tether extrusion, oligonucleotide stretching, and extraction of individual lipids from surfactant-monolayer surfaces of microbubbles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.