ArticleBiophysical journal2009
UVA generates pyrimidine dimers in DNA directly.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 162 citations in OpenAlex.
- SEM mapping of sequence-specific protein-DNA interactions on long DNA molecules.Nucleic acids research · 2026Article
- Linking UV-induced DNA damage with base pair sequences.bioRxiv : the preprint server for biology · 2026Article
- Chitosan Nanoformulations of Mycosporine-like Amino Acid (MAA)-Rich Extracts fromInternational journal of molecular sciences · 2025Article
- Efficacy of ultraviolet-light emitting diodes in bacterial inactivation and DNA damage via sensitivity evaluation using multiple wavelengths and bacterial strains.Archives of microbiology · 2025Article
- Impact of ecological UV radiation on the photochemistry of nuclear DNA.Biophysical reviews · 2025Review
- Destructive effects of UVC radiation on Drosophila melanogaster: Mortality, fertility, mutations, and molecular mechanisms.PloS one · 2024Article
- Could violet-blue lights increase the bacteria resistance against ultraviolet radiation mediated by photolyases?Lasers in medical science · 2023Review
- Exploring a chemical scaffold for rapid and selective photoaffinity labelling of non-ribosomal peptide synthetases in living bacterial cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- UVA Radiation, DNA Damage, and Melanoma.ACS omega · 2022Review
- Expression of Scytonemin Biosynthesis Genes under Alternative Stress Conditions in the CyanobacteriumMicroorganisms · 2022Article
- Lignin Nanoparticles Deliver Novel Thymine Biomimetic Photo-Adducts with Antimelanoma Activity.International journal of molecular sciences · 2022Article
- Effect of Cold Atmospheric Plasma on Epigenetic Changes, DNA Damage, and Possibilities for Its Use in Synergistic Cancer Therapy.International journal of molecular sciences · 2021Review
- Atomic force microscopy-A tool for structural and translational DNA research.APL bioengineering · 2021Review
- Ultraviolet light screen using cholesteric liquid crystal capsules on the basis of selective reflection.RSC advances · 2021Article
- Identification of unknown acid-resistant genes of oral microbiotas in patients with dental caries using metagenomics analysis.AMB Express · 2021Article
- Solar ultraviolet-induced DNA damage response: Melanocytes story in transformation to environmental melanomagenesis.Environmental and molecular mutagenesis · 2020Review
- Mechanisms of UV-induced mutations and skin cancer.Genome instability & disease · 2020Article
- Predicting attenuation of solar radiation (UV-B, UV-A and PAR) in waste stabilization ponds under Sahelian climatic conditions.Environmental science and pollution research international · 2018Article
- Sequence-programmable covalent bonding of designed DNA assemblies.Science advances · 2018Article
- Fabrication and characterization of UV-emitting nanoparticles as novel radiation sensitizers targeting hypoxic tumor cells.Optical Materials · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In addition, the relative amounts of various types of UVA lesions and their mutagenic significance are also a subject of debate. Here, we exploit atomic force microscopy (AFM) imaging of individual DNA molecules, alone and in complexes with a suite of DNA repair enzymes and antibodies, to directly quantify UVA damage and reexamine its basic mechanisms at a single-molecule level. By combining the activity of endonuclease IV and T4 endonuclease V on highly purified and UVA-irradiated pUC18 plasmids, we show by direct AFM imaging that UVA produces a significant amount of abasic sites and cyclobutane pyrimidine dimers (CPDs). However, we find that only approximately 60% of the T4 endonuclease V-sensitive sites, which are commonly counted as CPDs, are true CPDs; the other 40% are abasic sites. Most importantly, our results obtained by AFM imaging of highly purified native and synthetic DNA using T4 endonuclease V, photolyase, and anti-CPD antibodies strongly suggest that CPDs are produced by UVA directly. Thus, our observations contradict the predominant view that as-yet-unidentified photosensitizers are required to transfer the energy of UVA to DNA to produce CPDs. Our results may help to resolve the long-standing controversy about the origin of UVA-produced CPDs in DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.