ArticleBiophysical journal2009
Mechanical distortion of single actin filaments induced by external force: detection by fluorescence imaging.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- SurFlex microscopy: Measuring flexibility of surface-tethered biomolecules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Hierarchical Mechanotransduction System: From Macro to Micro.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Vinculin Y822 is an important determinant of ligand binding.Journal of cell science · 2023Article
- Flagella-like beating of actin bundles driven by self-organized myosin waves.Nature physics · 2022Article
- Phosphorylation of the small heat shock protein HspB1 regulates cytoskeletal recruitment and cell motility.Molecular biology of the cell · 2022Article
- Mechanochemical coupling of formin-induced actin interaction at the level of single molecular complex.Biomechanics and modeling in mechanobiology · 2020Article
- Force-history dependence and cyclic mechanical reinforcement of actin filaments at the single molecular level.Journal of cell science · 2019Article
- Article
- Construction of Chitosan-Zn-Based Electrochemical Biosensing Platform for Rapid and Accurate Assay of Actin.Sensors (Basel, Switzerland) · 2018Article
- Mechanical signals activate p38 MAPK pathway-dependent reinforcement of actin via mechanosensitive HspB1.Molecular biology of the cell · 2017Article
- Biphasic Effect of Profilin Impacts the Formin mDia1 Force-Sensing Mechanism in Actin Polymerization.Biophysical journal · 2017Article
- Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium.Journal of microbiology (Seoul, Korea) · 2017Article
- Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.Experimental cell research · 2016Review
- Molecular Mechanoneurobiology: An Emerging Angle to Explore Neural Synaptic Functions.BioMed research international · 2015Review
- Review
- Actin filaments as tension sensors.Current biology : CB · 2012Review
- Article
- Actin filaments function as a tension sensor by tension-dependent binding of cofilin to the filament.The Journal of cell biology · 2011Article
- Microfluidics pushes forward microscopy analysis of actin dynamics.Bioarchitecture · 2011Article
- The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends.Nature cell biology · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin is a major component of the cytoskeleton that transmits mechanical stress in both muscle and nonmuscle cells. As the first step toward developing a "bio-nano strain gauge" that would be able to report the mechanical stress imposed on an actin filament, we quantitatively examined the fluorescence intensity of dyes attached to single actin filaments under various tensile forces (5-20 pN). Tensile force was applied via two optically trapped plastic beads covalently coated with chemically modified heavy meromyosin molecules that were attached to both end regions of an actin filament. As a result, we found that the fluorescence intensity of an actin filament, where 20% of monomers were labeled with tetramethylrhodamine (TMR)-5-maleimide at Cys(374) and the filamentous structure was stabilized with nonfluorescent phalloidin, decreased by approximately 6% per 10 pN of the applied force, whereas the fluorescence intensity of an actin filament labeled with either BODIPY TMR cadaverin-iodoacetamide at Cys(374) or rhodamine-phalloidin showed only an approximately 2% decrease per 10 pN of the applied force. On the other hand, spectroscopic measurements of actin solutions showed that the fluorescence intensity of TMR-actin increased 1.65-fold upon polymerization (G-F transformation), whereas that of BODIPY-actin increased only 1.06-fold. These results indicate that the external force distorts the filament structure, such that the microenvironment around Cys(374) approaches that in G-actin. We thus conclude that the fluorescent dye incorporated into an appropriate site of actin can report the mechanical distortion of the binding site, which is a necessary condition for the bio-nano strain gauge.
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