ArticleJournal of the American Society for Mass Spectrometry2009
Measurement of neuropeptides in crustacean hemolymph via MALDI mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 54 citations in OpenAlex.
- Mass Spectrometry-Based Peptidomics for the Discovery and Profiling of Endogenous Peptides in Crustacean Hemolymph.ACS omega · 2026Article
- Unveiling the Impact of Rapeseed Meal on Feeding Behavior and Anorexigenic Endocrine inAnimals : an open access journal from MDPI · 2024Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus.Journal of visualized experiments : JoVE · 2022Article
- Mass spectrometry profiling and quantitation of changes in circulating hormones secreted over time in Cancer borealis hemolymph due to feeding behavior.Analytical and bioanalytical chemistry · 2022Article
- Developing mass spectrometry for the quantitative analysis of neuropeptides.Expert review of proteomics · 2021Review
- Exploring the Sexual Dimorphism of Crustacean Neuropeptide Expression UsingJournal of proteome research · 2021Article
- Multifaceted Mass Spectrometric Investigation of Neuropeptide Changes in Atlantic Blue Crab, Callinectes sapidus, in Response to Low pH Stress.Journal of proteome research · 2019Article
- To what extent may peptide receptor gene diversity/complement contribute to functional flexibility in a simple pattern-generating neural network?Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2019Article
- Subcellular Peptide Localization in Single Identified Neurons by Capillary Microsampling Mass Spectrometry.Scientific reports · 2018Article
- A high resolution atmospheric pressure matrix-assisted laser desorption/ionization-quadrupole-orbitrap MS platform enables in situ analysis of biomolecules by multi-mode ionization and acquisition.Analytica chimica acta · 2018Article
- Removal of endogenous neuromodulators in a small motor network enhances responsiveness to neuromodulation.Journal of neurophysiology · 2017Article
- Expression and distribution of neuropeptides in the nervous system of the crab Carcinus maenas and their roles in environmental stress.Proteomics · 2015Article
- Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides.Biochimica et biophysica acta · 2015Review
- Mass spectrometric measurement of neuropeptide secretion in the crab, Cancer borealis, by in vivo microdialysis.The Analyst · 2015Article
- Neuropeptide receptor transcript expression levels and magnitude of ionic current responses show cell type-specific differences in a small motor circuit.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Quantitative neuropeptidomics study of the effects of temperature change in the crab Cancer borealis.Journal of proteome research · 2014Article
- In situ identification and mapping of neuropeptides from the stomatogastric nervous system of Cancer borealis.Rapid communications in mass spectrometry : RCM · 2014Article
- A multi-scale strategy for discovery of novel endogenous neuropeptides in the crustacean nervous system.Journal of proteomics · 2013Article
- Motor circuit-specific burst patterns drive different muscle and behavior patterns.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Neuropeptides are often released into circulatory fluid (hemolymph) to act as circulating hormones and regulate many physiological processes. However, the detection of these low-level peptide hormones in circulation is often complicated by high salt interference and rapid degradation of proteins and peptides in crude hemolymph extracts. In this study, we systematically evaluated three different neuropeptide extraction protocols and developed a simple and effective hemolymph preparation method suitable for MALDI MS profiling of neuropeptides by combining acid-induced abundant protein precipitation/depletion, ultrafiltration, and C(18) micro-column desalting. In hemolymph samples collected from the crab Cancer borealis, several secreted neuropeptides have been detected, including members from at least five neuropeptide families, such as RFamide, allatostatin, orcokinin, tachykinin-related peptide (TRP), and crustacean cardioactive peptide (CCAP). Furthermore, two TRPs were detected in the hemolymph collected from food-deprived animals, suggesting the potential role of these neuropeptides in feeding regulation. In addition, a novel peptide with a Lys-Phe-amide C-terminus was identified and de novo sequenced directly from the Cancer borealis hemolymph sample. To better characterize the hemolymph peptidome, we also identified several abundant peptide signals in C. borealis hemolymph that were assigned to protein degradation products. Collectively, our study describes a simple and effective sample preparation method for neuropeptide analysis directly from crude crustacean hemolymph. Numerous endogenous neuropeptides were detected, including both known ones and new peptides whose functions remain to be characterized.
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Registered trials
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