ArticleCurrent microbiology2009
Monitoring oxidative stress and DNA damage induced by heavy metals in yeast expressing a redox-sensitive green fluorescent protein.
Article in Current microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase.PloS one · 2023Article
- In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook.World journal of microbiology & biotechnology · 2021Review
- Methylglyoxal-Scavenging Enzyme Activities Trigger Erythroascorbate Peroxidase and Cytochrome c Peroxidase in Glutathione-DepletedJournal of microbiology and biotechnology · 2021Article
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Live-cell imaging approaches for the investigation of xenobiotic-induced oxidant stress.Biochimica et biophysica acta · 2016Review
- Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii.Environmental science and pollution research international · 2015Article
- Redox-sensitive GFP2: use of the genetically encoded biosensor of the redox status in the filamentous fungus Botrytis cinerea.Molecular plant pathology · 2012Article
- Sirtuins as regulators of the yeast metabolic network.Frontiers in pharmacology · 2012Article
- A bacterial biosensor for oxidative stress using the constitutively expressed redox-sensitive protein roGFP2.Sensors (Basel, Switzerland) · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) has been proposed to play an important role in heavy metal-associated toxicity and pathology. Conventional methods for determining ambient redox state in cells are usually labor-intensive, precluding real-time or single-cell monitoring changes in intracellular redox poise resulting from either metabolic processes or environmental influences. Redox-sensitive green fluorescent protein (roGFP) was expressed in Saccharomyces cerevisiae and recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions. roGFP expressed in yeast responded not only to typical membrane-permeant oxidants and reductants, but also to toxicological metal ion-induced intracellular redox changes. Moreover, exposure of yeast cells to NaAsO(2) or Pb(NO(3))(2) at concentrations that induced redox changes reported by roGFP caused up to two- to three-fold increases in DNA mutation frequency. This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals significantly reduced the mutation rate as well as delayed the cell death.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.