ArticleTransgenic research2009
Tissue specific expression of potent insecticidal, Allium sativum leaf agglutinin (ASAL) in important pulse crop, chickpea (Cicer arietinum L.) to resist the phloem feeding Aphis craccivora.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 111 citations in OpenAlex.
- A method for maintaining the release of co-suppression and maximally restoring the RDR6 expression.Plant cell reports · 2025Article
- Review
- Chickpea: Its Origin, Distribution, Nutrition, Benefits, Breeding, and Symbiotic Relationship withPlants (Basel, Switzerland) · 2024Review
- Chickpea (Plants (Basel, Switzerland) · 2022Review
- A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2.Virus research · 2022Article
- Article
- Elicitation of biomolecules as host defense arsenals during insect attacks on tea plants (Camellia sinensis (L.) Kuntze).Applied microbiology and biotechnology · 2021Review
- Overview of the Structure⁻Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi.International journal of molecular sciences · 2019Review
- An Update on Genetic Modification of Chickpea for Increased Yield and Stress Tolerance.Molecular biotechnology · 2018Review
- Expression of Colocasia esculenta tuber agglutinin in Indian mustard provides resistance against Lipaphis erysimi and the expressed protein is non-allergenic.Plant cell reports · 2018Article
- Article
- Characterization of chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Characterization of an Insecticidal Protein from Withania somnifera Against Lepidopteran and Hemipteran Pest.Molecular biotechnology · 2018Article
- Whitefly and aphid inducible promoters of Arabidopsis thaliana L.Journal of genetics · 2018Article
- Viruses and Phytoparasitic Nematodes ofFrontiers in plant science · 2018Review
- Expression of a Chimeric Gene Encoding Insecticidal Crystal Protein Cry1Aabc ofFrontiers in plant science · 2017Article
- Monitoring the efficacy of mutated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani.BMC biotechnology · 2016Article
- Strength, Stability, and cis-Motifs of In silico Identified Phloem-Specific Promoters in Brassica juncea (L.).Frontiers in plant science · 2016Article
- Identification of differentially expressed genes related to aphid resistance in cucumber (Cucumis sativus L.).Scientific reports · 2015Article
- Development of selectable marker free, insect resistant, transgenic mustard (Brassica juncea) plants using Cre/lox mediated recombination.BMC biotechnology · 2013Article
Corrections and comments
- Expression of concern
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phloem sap-sucking hemipteran insect, Aphis craccivora, commonly known as cowpea aphid, cause major yield loss of important food legume crop chickpea. Among different plant lectins Allium sativum leaf agglutinin (ASAL), a mannose binding lectin was found to be potent antifeedant for sap sucking insect A. craccivora. Present study describes expression of ASAL in chickpea through Agrobacterium-mediated transformation of "single cotyledon with half embryo" explant. ASAL was expressed under the control of CaMV35S promoter for constitutive expression and phloem specific rolC promoter for specifically targeting the toxin at feeding site, using pCAMBIA2301 vector containing plant selection marker nptII. Southern blot analysis demonstrated the integration and copy number of chimeric ASAL gene in chickpea and its inheritance in T(1) and T(2) progeny plants. Expression of ASAL in T(0) and T(1) plants was confirmed through northern and western blot analysis. The segregation pattern of ASAL transgene was observed in T(1) progenies, which followed the 3:1 Mendelian ratio. Enzyme linked immunosorbant assay (ELISA) determined the level of ASAL expression in different transgenic lines in the range of 0.08-0.38% of total soluble protein. The phloem tissue specific expression of ASAL gene driven by rolC promoter has been monitored by immunolocalization analysis of mature stem sections. Survival and fecundity of A. craccivora decreased to 11-26% and 22-42%, respectively when in planta bioassay conducted on T(1) plants compared to untransformed control plant which showed 85% survival. Thus, through unique approach of phloem specific expression of novel insecticidal lectin (ASAL), aphid resistance has been successfully achieved in chickpea.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.