ArticleTransgenic research2009
Extensive homologous recombination between introduced and native regulatory plastid DNA elements in transplastomic plants.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The First Introduction of an Exogenous 5' Untranslated Region for Control of Plastid Transgene Expression in Chlamydomonas reinhardtii.Molecular biotechnology · 2025Article
- Twelve newly assembled jasmine chloroplast genomes: unveiling genomic diversity, phylogenetic relationships and evolutionary patterns among Oleaceae and Jasminum species.BMC plant biology · 2024Article
- Chloroplast genomes of fourFrontiers in plant science · 2023Article
- Expression strategies for the efficient synthesis of antimicrobial peptides in plastids.Nature communications · 2022Article
- Mini-synplastomes for plastid genetic engineering.Plant biotechnology journal · 2022Article
- Mitigation of deleterious phenotypes in chloroplast-engineered plants accumulating high levels of foreign proteins.Biotechnology for biofuels · 2021Article
- Plastome Evolution in the Hyperdiverse GenusFrontiers in plant science · 2021Article
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.Methods in molecular biology (Clifton, N.J.) · 2021Review
- A Repertory of Rearrangements and the Loss of an Inverted Repeat Region in Passiflora Chloroplast Genomes.Genome biology and evolution · 2020Article
- The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis.Plant physiology · 2020Article
- Unprecedented Intraindividual Structural Heteroplasmy in Eleocharis (Cyperaceae, Poales) Plastomes.Genome biology and evolution · 2020Article
- Directed Repeats Co-occur with Few Short-Dispersed Repeats in Plastid Genome of a Spikemoss, Selaginella vardei (Selaginellaceae, Lycopodiopsida).BMC genomics · 2019Article
- Expression of VGRNb-PE immunotoxin in transplastomic lettuce (Lactuca sativa L.).Plant molecular biology · 2018Article
- The Repeat Sequences and Elevated Substitution Rates of the ChloroplastFrontiers in plant science · 2018Article
- Genus-Wide Screening Reveals Four Distinct Types of Structural Plastid Genome Organization in Pelargonium (Geraniaceae).Genome biology and evolution · 2017Article
- Towards engineering carboxysomes into C3 plants.The Plant journal : for cell and molecular biology · 2016Article
- Predominant and substoichiometric isomers of the plastid genome coexist within Juniperus plants and have shifted multiple times during cupressophyte evolution.Genome biology and evolution · 2014Article
- Article
- Mechanisms of functional and physical genome reduction in photosynthetic and nonphotosynthetic parasitic plants of the broomrape family.The Plant cell · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination within plastids directs plastid genome transformation for foreign gene expression and study of plastid gene function. Though transgenes are generally efficiently targeted to their desired insertion site, unintended homologous recombination events have been observed during plastid transformation. To understand the nature and abundance of these recombination events, we analyzed transplastomic tobacco lines derived from three different plastid transformation vectors utilizing two different loci for foreign gene insertion. Two unintended recombinant plastid DNA species were formed from each regulatory plastid DNA element included in the transformation vector. Some of these recombinant DNA species accumulated to as much as 10-60% of the amount of the desired integrated transgenic sequence in T0 plants. Some of the recombinant DNA species undergo further, "secondary" recombination events, resulting in an even greater number of recombinant plastid DNA species. The abundance of novel recombinant DNA species was higher in T0 plants than in T1 progeny, indicating that the ancillary recombination events described here may have the greatest impact during selection and regeneration of transformants. A line of transplastomic tobacco was identified containing an antibiotic resistance gene unlinked from the intended transgene insertion as a result of an unintended recombination event, indicating that the homologous recombination events described here may hinder efficient recovery of plastid transformants containing the desired transgene.
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