ReviewIn vitro cellular & developmental biology. Animal
Applications and potential uses of fish gill cell lines: examples with RTgill-W1.
Review in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Establishment and characterisation of the first cell line from the giant Amazonian fish Arapaima gigas and its susceptibility to Tilapia Lake virus (TiLV).Fish physiology and biochemistry · 2026Article
- Improve your success with fish cell lines-small things that matter.In vitro cellular & developmental biology. Animal · 2026Review
- Towards Animal-Free Toxicology: Establishment of Two Larval Brown Trout Cell Lines for Environmental Risk Assessment.Toxics · 2025Article
- Isolation and characterisation of two epithelial-like cell lines from the gills of Chrysophrys auratus (Australasian snapper) and Oncorhynchus tshawytscha (Chinook salmon) and their use in aquatic toxicology.In vitro cellular & developmental biology. Animal · 2025Article
- Application of Novel Gill Cell Line from Lates calcarifer for Recognizing Metals Using Probes.Biological trace element research · 2025Article
- Establishment and identification of the gill cell line from the blunt snout bream (Megalobrama amblycephala) and its application in studying gill remodeling under hypoxia.Fish physiology and biochemistry · 2024Article
- Effectiveness of functional ingredients to enhance gill disease in Atlantic salmon (Salmo salar, L.).PloS one · 2024Article
- An established kidney cell line from humpback grouper (Cromileptes altivelis) and its susceptibility to bacteria and heavy metals.Fish physiology and biochemistry · 2022Article
- Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878).Fish physiology and biochemistry · 2022Article
- Review
- Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles.Nanomaterials (Basel, Switzerland) · 2021Article
- Proteome Analysis of Whole-Body Responses in Medaka Experimentally Exposed to Fish-Killing DinoflagellateInternational journal of molecular sciences · 2021Article
- Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Cytotoxicity of Mycotoxins Frequently Present in Aquafeeds to the Fish Cell Line RTGill-W1.Toxins · 2021Article
- Differential Exoproteome and Biochemical Characterisation ofMicroorganisms · 2021Article
- Molecular and biochemical characterization of the bicarbonate-sensing soluble adenylyl cyclase from a bony fish, the rainbow troutInterface focus · 2021Article
- Manganese dioxide nanosheets induce mitochondrial toxicity in fish gill epithelial cells.Nanotoxicology · 2021Article
- Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion.International journal of environmental research and public health · 2021Article
- Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus.Scientific reports · 2020Article
- Chemical and Colloidal Dynamics of MnOSmall (Weinheim an der Bergstrasse, Germany) · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gills are unique structures involved in respiration and osmoregulation in piscinids as well as in many aquatic invertebrates. The availability of the trout-derived gill cell line, RTgill-W1, is beginning to make impacts in fish health and toxicology. These cells are available from the American Type Culture Collection as ATCC CRL 2523. The cells have an epithelioid morphology and form tight monolayer sheets that can be used for testing epithelial resistance. The cells can be grown in regular tissue culture surfaces or in transwell membranes in direct contact with water on their apical surfaces. The ability of RTgill-W1 to withstand hypo- and hyper-osmotic conditions and their optimal growth capacity at room temperature, make these cells ideal sentinel models for in vitro aquatic toxicology as well as model systems to study fish gill function and gill diseases. RTgill-W1 support growth of paramyxoviruses and orthomyxoviruses like salmon anemia virus. RTgill-W1 also support growth of Neoparamoeba pemaquidensis, the causative agent of amoebic gill disease. The cells have been used to understand mechanisms of toxicity, ranking the potencies of toxicants, and evaluating the toxicity of environmental samples. These cells are also valuable for high throughput toxicogenomic and toxicoproteomic studies which are easier to achieve with cell lines than with whole organisms. RTgill-W1 cell line could become a valuable complement to whole animal studies and in some cases as gill replacements in aquatic toxicology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.