ArticleInternational journal of legal medicine2010
Time-dependent expression of skeletal muscle troponin I mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation.
Article in International journal of legal medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- State-of-the-Art on Wound Vitality Evaluation: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Screening criteria of mRNA indicators for wound age estimation.Forensic sciences research · 2022Article
- Gill and Liver Transcript Expression Changes Associated With Gill Damage in Atlantic Salmon (Frontiers in immunology · 2022Article
- Vitality and wound-age estimation in forensic pathology: review and future prospects.Forensic sciences research · 2020Review
- Temporal expression of wound healing-related genes inform wound age estimation in rats after a skeletal muscle contusion: a multivariate statistical model analysis.International journal of legal medicine · 2020Article
- Forensic aspects of gene expression signatures for age determination in bruises as evaluated in an experimental porcine model.Forensic science, medicine, and pathology · 2017Article
- A novel, comprehensive, and reproducible porcine model for determining the timing of bruises in forensic pathology.Forensic science, medicine, and pathology · 2016Article
- Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age.International journal of legal medicine · 2016Article
- The time-dependent expression of α7nAChR during skeletal muscle wound healing in rats.International journal of legal medicine · 2014Article
- Time-dependent expression of SNAT2 mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation.Forensic science, medicine, and pathology · 2013Article
- Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR.International journal of legal medicine · 2012Article
- Time-dependent expression and distribution of monoacylglycerol lipase during the skin-incised wound healing in mice.International journal of legal medicine · 2011Article
- Estimating wound age: looking into the future.International journal of legal medicine · 2010Review
- The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats.International journal of legal medicine · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To estimate the age of skeletal muscle contusion, the expression of troponin I mRNA in contused skeletal muscle of rats was detected using real-time polymerase chain reaction (PCR). A total of 51 Sprague-Dawley male rats were divided into control and contusion groups, and another nine rats received contusion injury after death. At 0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion, the rats were killed with a lethal dose of pentobarbital. Total RNA was isolated from muscle specimens using the SV Total RNA Isolation System and reverse transcribed into first-strand cDNA. Sequence-specific primers were then used to conduct real-time PCR to analyze the expression levels of sTnI mRNA. At 0.5, 1, and 6 h after contusion, the expression levels of sTnI mRNA decreased to 78.17% (P < 0.05), 41.58% (P < 0.05), and 32.13% of that in the control group, respectively. However, there were no significant changes in the expression levels of sTnI mRNA from 6 to 36 h (P > 0.05) after contusion when normalized to RpL32 expression. The expression levels of sTnI mRNA in the normal and contused skeletal muscle of postmortem rats were about 70% of that in the control group (P < 0.05), and no significant changes in the expression levels of sTnI mRNA in the postmortem contusion group were noted among different time points after injury. This result suggests that determination of sTnI mRNA levels by real-time PCR is useful for the estimation of wound age.
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