ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Genetic and physical mapping of new EST-derived SSRs on the A and B genome chromosomes of wheat.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed.
- Dissecting the genetic basis of resistance to Soil-borne cereal mosaic virus (SBCMV) in durum wheat by bi-parental mapping and GWAS.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Datasets for genetic diversity assessment in a collection of wild and cultivated pomegranates (Data in brief · 2023Article
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- Phenotypic characterization of the glossy1 mutant and fine mapping of GLOSSY1 in common wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- From Genetic Maps to QTL Cloning: An Overview for Durum Wheat.Plants (Basel, Switzerland) · 2021Review
- Functional Validation ofInternational journal of molecular sciences · 2020Article
- The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Transcriptome Sequencing and Development of Novel Genic SSR Markers FromFrontiers in genetics · 2020Article
- Physical information of 2705 PCR-based molecular markers and the evaluation of their potential use in wheat.Journal of genetics · 2019Article
- MusatransSSRDB (a transcriptome derived SSR database) - An advanced tool for banana improvement.Journal of biosciences · 2019Article
- Article
- High-density genetic mapping of a major QTL for resistance to multiple races of loose smut in a tetraploid wheat cross.PloS one · 2018Article
- Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Identification and Characterization of the Sucrose Synthase 2 Gene (Sus2) in Durum Wheat.Frontiers in plant science · 2016Article
- Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.PloS one · 2016Article
- Characterization of recombinants of the Aegilops peregrina-derived Lr59 translocation of common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Comparative fine mapping of the Wax 1 (W1) locus in hexaploid wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus.BioMed research international · 2015Article
- A consensus framework map of durum wheat (Triticum durum Desf.) suitable for linkage disequilibrium analysis and genome-wide association mapping.BMC genomics · 2014Article
- Characterization of EST-SSR markers in durum wheat EST library and functional analysis of SSR-containing EST fragments.Molecular genetics and genomics : MGG · 2014Article
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Abstract
The availability of genetic maps and phenotypic data of segregating populations allows to localize and map agronomically important genes, and to identify closely associated molecular markers to be used in marker-assisted selection and positional cloning. The objective of the present work was to develop a durum wheat intervarietal genetic and physical map based on genomic microsatellite or genomic simple sequence repeats (gSSR) markers and expressed sequence tag (EST)-derived microsatellite (EST-SSR) markers. A set of 122 new EST-SSR loci amplified by 100 primer pairs was genetically mapped on the wheat A and B genome chromosomes. The whole map also comprises 149 gSSR markers amplified by 120 primer pairs used as anchor chromosome loci, two morphological markers (Black colour, Bla1, and spike glaucousness, Ws) and two seed storage protein loci (Gli-A2 and Gli-B2). The majority of SSR markers tested (182) was chromosome-specific. Out of 275 loci 241 loci assembled in 25 linkage groups assigned to the chromosomes of the A and B genome and 34 remained unlinked. A higher percentage of markers (54.4%), localized on the B genome chromosomes, in comparison to 45.6% distributed on the A genome. The whole map covered 1,605 cM. The B genome accounted for 852.2 cM of genetic distance; the A genome basic map spanned 753.1 cM with a minimum length of 46.6 cM for chromosome 5A and a maximum of 156.2 cM for chromosome 3A and an average value of 114.5 cM. The primer sets that amplified two or more loci mapped to homoeologous as well as to non-homoeologous sites. Out of 241 genetically mapped loci 213 (88.4%) were physically mapped by using the nulli-tetrasomic, ditelosomic and a stock of 58 deletion lines dividing the A and B genome chromosomes in 94 bins. No discrepancies concerning marker order were observed but the cytogenetic maps revealed in some cases small genetic distance covered large physical regions. Putative function for mapped SSRs were assigned by searching against GenBank nonredundant database using TBLASTX algorithms.
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