ArticleJournal of bone and mineral metabolism2009
Transient dynamic actin cytoskeletal change stimulates the osteoblastic differentiation.
Article in Journal of bone and mineral metabolism, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Murine model of high bone mass osteogenesis imperfecta exhibits bone matrix hyper-mineralization, misaligned mineral crystals, and altered osteoblast differentiation.Bone research · 2026Article
- Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton's Jelly Mesenchymal Stem Cells.Pharmaceuticals (Basel, Switzerland) · 2023Article
- The effects of 808-nm near-infrared laser light irradiation on actin cytoskeleton reorganization in bone marrow mesenchymal stem cells.Cell and tissue research · 2021Article
- Concentrated growth factor promotes proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells in vitro.Journal of orthopaedic surgery and research · 2019Article
- Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2019Article
- Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation.Nature communications · 2016Article
- Pyrophosphate Stimulates Differentiation, Matrix Gene Expression and Alkaline Phosphatase Activity in Osteoblasts.PloS one · 2016Article
- Connectivity Map-based discovery of parbendazole reveals targetable human osteogenic pathway.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Microstructured zirconia surfaces modulate osteogenic marker genes in human primary osteoblasts.Journal of materials science. Materials in medicine · 2015Article
- RhoA controls Wnt upregulation on microstructured titanium surfaces.BioMed research international · 2014Article
- Activin A suppresses osteoblast mineralization capacity by altering extracellular matrix (ECM) composition and impairing matrix vesicle (MV) production.Molecular & cellular proteomics : MCP · 2013Article
- Destrin deletion enhances the bone loss in hindlimb suspended mice.European journal of applied physiology · 2013Article
- Tanshinol attenuates the deleterious effects of oxidative stress on osteoblastic differentiation via Wnt/FoxO3a signaling.Oxidative medicine and cellular longevity · 2013Article
- Actin microfilament mediates osteoblast Cbfa1 responsiveness to BMP2 under simulated microgravity.PloS one · 2013Article
- Inhibition of Rac and ROCK signalling influence osteoblast adhesion, differentiation and mineralization on titanium topographies.PloS one · 2013Article
- L-type calcium channel activity in osteoblast cells is regulated by the actin cytoskeleton independent of protein trafficking.Journal of bone and mineral metabolism · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamic cytoskeletal changes appear to be one of intracellular signals that control cell differentiation. To test this hypothesis, we examined the effects of short-term actin cytoskeletal changes on osteoblastic differentiation. We found an actin polymerization interfering reagent, cytochalasin D, promoted osteoblastic differentiation in mouse preosteoblastic MC3T3-E1 cells. We also found that these effects were mediated by the protein kinase D (PKD) pathway. Short-term cytochalasin D treatment increased alkaline phosphatase (ALP) activity, osteocalcin (OCN) secretion, and mineralization of the extracellular matrix in MC3T3-E1 cells, with temporary changes in actin cytoskeleton. Furthermore, the disruption of actin cytoskeleton induced phosphorylation of 744/748 serine within the activation loop of PKD in a dose-dependent manner. The protein kinase C (PKC)/PKD inhibitor Go6976 suppressed cytochalasin D-induced acceleration of osteoblastic differentiation, whereas Go6983, a specific inhibitor of conventional PKCs, did not. Involvement of PKD signaling was confirmed by using small interfering RNA to knock down PKD. In addition, another actin polymerization interfering reagent, latrunculin B, also stimulated ALP activity and OCN secretion with PKD activation. On the other hand, the present data suggested that transient dynamic actin cytoskeletal reorganization could be a novel cellular signal that directly stimulated osteoblastic differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.