Evidence map›Paper›PMID 19183794›Full record

ArticleJournal of biomolecular techniques : JBT2008

Aptamer selection express: a novel method for rapid single-step selection and sensing of aptamers.

Maomian Fan, Shelly Roper McBurnett, Carrie J Andrews, Amity M Allman, John G Bruno, Johnathan L Kiel

Abstract readEvaluation Study
In one paragraph

Article in Journal of biomolecular techniques : JBT, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Aptasensors versus immunosensors-Which will prevail?Engineering in life sciences · 2022
    Review
  4. Article
  5. Updates on Aptamer Research.International journal of molecular sciences · 2019
    Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Maomian FanAir Force Research Laboratories, RHPC, Brooks City-Base, TX, USA. maomian.fan@brooks.af.mil
Shelly Roper McBurnett
Carrie J Andrews
Amity M Allman
John G Bruno
Johnathan L Kiel

Funding

Nuclease-Resistant Aptamers to Botox and Anthrax ToxinsR43AI058561 · NIAID · OPERATIONAL TECHNOLOGIES CORPORATION · PI BRUNO, JOHN G · 2004 to 2004
$100k
NIAID NIH HHS 1R43AI058561-01NIAID NIH HHS R43 AI058561
6 · The paper itself

Abstract

Here we describe a new DNA capture element (DCE) sensing system, based on the quenching and dequenching of a double-stranded aptamer. This system shows very good sensitivity and thermal stability. While quenching, dequenching, and separating the DCE systems made from different aptamers (all selected by SELEX), an alternative method to rapidly select aptamers was developed-the Aptamer Selection Express (ASExp). This process has been used to select aptamers against different types of targets (Bacillus anthracis spores, Bacillus thuringiensis spores, MS-2 bacteriophage, ovalbumin, and botulinum neurotoxin). The DCE systems made from botulinum neurotoxin aptamers selected by ASExp have been investigated. The results of this investigation indicate that ASExp can be used to rapidly select aptamers for the DCE sensing system.

Indexed as

AnimalsAptamers, NucleotideBacillus anthracisBase SequenceBiotechnologyBotulinum ToxinsLevivirusOvalbuminPolymerase Chain ReactionSELEX Aptamer TechniqueSpores, BacterialAptamers, NucleotideBotulinum ToxinsOvalbuminaptamerASExpDCESELEX

Identifiers

PMID19183794
PMCPMC2628068

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.