ArticleMicrobial cell factories2009
Metabolite profiling studies in Saccharomyces cerevisiae: an assisting tool to prioritize host targets for antiviral drug screening.
Article in Microbial cell factories, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Extraction of Extracellular Matrix in Static and DynamicFrontiers in microbiology · 2019Article
- Metabonomics onEmerging microbes & infections · 2019Article
- Metabolomic Analysis and Mode of Action of Metabolites of Tea Tree Oil Involved in the Suppression ofFrontiers in microbiology · 2017Article
- Myxobacteria: natural pharmaceutical factories.Microbial cell factories · 2012Article
- Yeast cell factory: fishing for the best one or engineering it?Microbial cell factories · 2009Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cellular proteins Pat1p, Lsm1p, and Dhh1p are required for the replication of some positive-strand viruses and therefore are potential targets for new antiviral drugs. To prioritize host targets for antiviral drug screening a comparative metabolome analysis in Saccharomyces cerevisiae reference strain BY4742 Matalpha his3Delta1 leu2Delta0 lys2Delta0 ura3Delta0 and deletion strains pat1Delta, lsm1Delta and dhh1Delta was performed.
resultsGC/MS analysis permitted the quantification of 47 polar metabolites and the identification of 41 of them. Metabolites with significant variation between the strains were identified using partial least squares to latent structures discriminate analysis (PLS-DA). The analysis revealed least differences of pat1Delta to the reference strain as characterized by Euclidian distance of normalized peak areas. The growth rate and specific production rates of ethanol and glycerol were also most similar with this strain.
conclusionFrom these results we hypothesize that the human analog of yeast Pat1p is most likely the best drug target candidate.
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Registered trials
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