ArticleMolecular microbiology2009
Controlled degradation by ClpXP protease tunes the levels of the excision repair protein UvrA to the extent of DNA damage.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 1 citations in OpenAlex.
- Raphin-1 mediates the survival and sensitivity to radiation of pediatric-type diffuse high-grade glioma via phosphorylated eukaryotic initiation factor 2α-dependent and -independent processes.Molecular oncology · 2025Article
- Degron-controlled protein degradation inbioRxiv : the preprint server for biology · 2023Article
- Proteomic survey of the DNA damage response inJournal of bacteriology · 2023Article
- Proteomic survey of the DNA damage response inbioRxiv : the preprint server for biology · 2023Article
- The XPA Protein-Life under Precise Control.Cells · 2022Review
- Protease-deficient SOS constitutive cells have RecN-dependent cell division phenotypes.Molecular microbiology · 2019Article
- Genome Plasticity ofInfection and immunity · 2018Article
- Mfd Protein and Transcription-Repair Coupling in Escherichia coli.Photochemistry and photobiology · 2017Review
- ClpXP protease targets long-lived DNA translocation states of a helicase-like motor to cause restriction alleviation.Nucleic acids research · 2014Article
- Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation.Proteome science · 2013Article
- The ClpP protease is required for the stress tolerance and biofilm formation in Actinobacillus pleuropneumoniae.PloS one · 2013Article
- Opposing effects of DNA on proteolysis of a replication initiator.Nucleic acids research · 2012Article
- Mycobacterium tuberculosis ClpP1 and ClpP2 function together in protein degradation and are required for viability in vitro and during infection.PLoS pathogens · 2012Article
- ClpXP, an ATP-powered unfolding and protein-degradation machine.Biochimica et biophysica acta · 2012Review
- Regulated proteolysis in Gram-negative bacteria--how and when?Nature reviews. Microbiology · 2011Review
- Destroying the ring: Freeing DNA from Ku with ubiquitin.FEBS letters · 2011Review
- Distinct properties of hexameric but functionally conserved Mycobacterium tuberculosis transcription-repair coupling factor.PloS one · 2011Article
- Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein.Nucleic acids research · 2011Article
- Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The IbpA and IbpB small heat-shock proteins are substrates of the AAA+ Lon protease.Molecular microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
UV irradiation damages DNA and activates expression of genes encoding proteins helpful for survival under DNA stress. These proteins are often deleterious in the absence of DNA damage. Here, we investigate mechanisms used to regulate the levels of DNA-repair proteins during recovery by studying control of the nucleotide excision repair (NER) protein UvrA. We show that UvrA is induced after UV irradiation and reaches maximum levels between approximately 20 and 120 min post UV. During post-UV recovery, UvrA levels decrease principally as a result of ClpXP-dependent protein degradation. The rate of UvrA degradation depends on the amount of unrepaired pyrimidine dimers present; this degradation rate is initially slow shortly after UV, but increases as damage is repaired. This increase in UvrA degradation as repair progresses is also influenced by protein-protein interactions. Genetic and in vitro experiments support the conclusion that UvrA-UvrB interactions antagonize degradation. In contrast, Mfd appears to act as an enhancer of UvrA turnover. Thus, our results reveal that a complex network of interactions contribute to tuning the level of UvrA in the cell in response to the extent of DNA damage and nicely mirror findings with excision repair proteins from eukaryotes, which are controlled by proteolysis in a similar manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.