ArticleMolecular microbiology2009
The archaeal RNA polymerase subunit P and the eukaryotic polymerase subunit Rpb12 are interchangeable in vivo and in vitro.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
- Deeply conserved and expressed small ORFs in the model beetle Tribolium castaneum.BMC biology · 2026Article
- Molecular Evolution of the Archaeal DNA-Dependent RNA Polymerase: Cooperative Changes in Subunit Composition and Specific Domains of Small Subunits.International journal of molecular sciences · 2026Article
- Promoter contacts by TFB and TFEα during archaeal transcription initiation.Transcription · 2026Article
- Structure of the recombinant RNA polymerase from African Swine Fever Virus.Nature communications · 2024Article
- Structure-Based Deep Mining Reveals First-Time Annotations for 46 Percent of the Dark Annotation Space of the 9,671-Member Superproteome of the Nucleocytoplasmic Large DNA Viruses.Journal of virology · 2020Article
- Multisubunit DNA-Dependent RNA Polymerases from Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions from the Age of Structure.Microbiology and molecular biology reviews : MMBR · 2017Review
- Analyses of in vivo interactions between transcription factors and the archaeal RNA polymerase.Methods (San Diego, Calif.) · 2015Article
- Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Article
- NanoRNAs: a class of small RNAs that can prime transcription initiation in bacteria.Journal of molecular biology · 2011Article
- Functional analysis of archaeal MBF1 by complementation studies in yeast.Biology direct · 2011Article
- Evolution of multisubunit RNA polymerases in the three domains of life.Nature reviews. Microbiology · 2011Review
- Fidelity in archaeal information processing.Archaea (Vancouver, B.C.) · 2010Review
- Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes.Nucleic acids research · 2010Article
- Archaeal RNA polymerase.Current opinion in structural biology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The general subunit of all three eukaryotic RNA polymerases, Rpb12, and subunit P of the archaeal enzyme show sequence similarities in their N-terminal zinc ribbon and some highly conserved residues in the C-terminus. We report here that archaeal subunit P under the control of a strong yeast promoter could complement the lethal phenotype of a RPB12 deletion mutant and that subunit Rpb12 from yeast can functionally replace subunit P during reconstitution of the archaeal RNA polymerase. The DeltaP enzyme is unable to form stable open complexes, but can efficiently extend a dinucleotide on a premelted template or RNA on an elongation scaffold. This suggests that subunit P is directly or indirectly involved in promoter opening. The activity of the DeltaP enzyme can be rescued by the addition of Rpb12 or subunit P to transcription reactions. Mutation of cysteine residues in the zinc ribbon impair the activity of the enzyme in several assays and this mutated form of P is rapidly replaced by wild-type P in transcription reactions. The conserved zinc ribbon in the N-terminus seems to be important for proper interaction of the complete subunit with other RNA polymerase subunits and a 17-amino-acid C-terminal peptide is sufficient to support all basic RNA polymerase functions in vitro.
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