ArticleNature cell biology2009
An ATM- and ATR-dependent checkpoint inactivates spindle assembly by targeting CEP63.
Article in Nature cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- Primary cilia in cancer: structures, functions, mechanisms, and therapeutic implications.Cancer biology & medicine · 2025Review
- Pharmaceutical inhibition of the Chk2 kinase mitigates cone photoreceptor degeneration in an iPSC model of Bardet-Biedl syndrome.iScience · 2025Article
- Extensive homologous recombination safeguards oocyte genome integrity in mammals.Nucleic acids research · 2025Article
- Molecular Link between DNA Damage Response and Microtubule Dynamics.International journal of molecular sciences · 2022Review
- Targeting Cell Cycle Checkpoint Kinases to Overcome Intrinsic Radioresistance in Brain Tumor Cells.Cancers · 2022Review
- Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1.Oncology reports · 2021Article
- p53 Activation in Genetic Disorders: Different Routes to the Same Destination.International journal of molecular sciences · 2021Review
- Sufu negatively regulates both initiations of centrosome duplication and DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The DNA damage response links human squamous proliferation with differentiation.The Journal of cell biology · 2020Article
- How Cells Handle DNA Breaks during Mitosis: Detection, Signaling, Repair, and Fate Choice.Cells · 2019Review
- Regulation of centriolar satellite integrity and its physiology.Cellular and molecular life sciences : CMLS · 2017Review
- Cip29 is phosphorylated following activation of the DNA damage response in Xenopus egg extracts.PloS one · 2017Article
- Maintaining Genome Stability in Defiance of Mitotic DNA Damage.Frontiers in genetics · 2016Review
- Review
- CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination.Nature communications · 2015Article
- Synthetic cytotoxicity: digenic interactions with TEL1/ATM mutations reveal sensitivity to low doses of camptothecin.Genetics · 2014Article
- Interplay between the DNA damage proteins MDC1 and ATM in the regulation of the spindle assembly checkpoint.The Journal of biological chemistry · 2014Article
- CEP proteins: the knights of centrosome dynasty.Protoplasma · 2013Review
- Copy number gains of FGFR1 and 3q chromosome in squamous cell carcinoma of the lung.Translational lung cancer research · 2013Review
- FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1.Biology open · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
Activation of the protein kinases ATM and ATR following chromosomal breakage prevents initiation of DNA replication and entry into mitosis. However, the effects of ATM and ATR activation in cells already progressing through mitosis are poorly understood. Here we report that ATM and ATR activation induced by DNA double-strand breaks (DSBs) inhibits centrosome-driven spindle assembly in Xenopus laevis mitotic egg extract and somatic cells, delaying mitotic progression. Using a cDNA expression library to screen for ATM and ATR substrates, we identified centrosomal protein CEP63 as an ATM and ATR target required for normal spindle assembly. ATM and ATR phosphorylate Xenopus CEP63 (XCEP63) on Ser 560 and promote its delocalization from the centrosome. Suppression of ATM and ATR activity or mutation of XCEP63 Ser 560 to Ala prevented spindle assembly defects. Consistently, inactivation of the CEP63 gene in avian DT40 cells impaired spindle assembly and prevented ATM- and ATR-dependent effects on mitosis. These data indicate that ATM and ATR control mitotic events in vertebrate cells by targeting CEP63 and centrosome dependent spindle assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.