ArticleEukaryotic cell2009
Isolation and characterization of Cryptococcus neoformans spores reveal a critical role for capsule biosynthesis genes in spore biogenesis.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 85 citations in OpenAlex.
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- Article
- Is Cryptococcus neoformans a pleomorphic fungus?Current opinion in microbiology · 2024Review
- Alternative isoforms and phase separation of Ref1 repress morphogenesis in Cryptococcus.Cell reports · 2024Article
- Illuminating the Cryptococcus neoformans species complex: unveiling intracellular structures with fluorescent-protein-based markers.Genetics · 2024Article
- Biogenesis, germination, and pathogenesis ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Vaccination with aInfection and immunity · 2023Article
- The Cryptococcus gattii species complex: Unique pathogenic yeasts with understudied virulence mechanisms.PLoS neglected tropical diseases · 2022Review
- A dissemination-prone morphotype enhances extrapulmonary organ entry by Cryptococcus neoformans.Cell host & microbe · 2022Article
- Article
- Methods for ManipulatingJournal of fungi (Basel, Switzerland) · 2021Review
- Article
- Cryptococcus neoformans: Sex, morphogenesis, and virulence.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Review
- Pleiotropy and epistasis within and between signaling pathways defines the genetic architecture of fungal virulence.PLoS genetics · 2021Article
- The LAMMER Kinase, LkhA, AffectsFrontiers in cellular and infection microbiology · 2021Article
- Assessing the virulence of Cryptococcus neoformans causing meningitis in HIV infected and uninfected patients in Vietnam.Medical mycology · 2020Article
- Centromere scission drives chromosome shuffling and reproductive isolation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Evolution of Sexual Reproduction and the Mating-Type Locus: Links to Pathogenesis ofAnnual review of genetics · 2019Review
- Spore Germination as a Target for Antifungal Therapeutics.Antimicrobial agents and chemotherapy · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Spores are essential particles for the survival of many organisms, both prokaryotic and eukaryotic. Among the eukaryotes, fungi have developed spores with superior resistance and dispersal properties. For the human fungal pathogens, however, relatively little is known about the role that spores play in dispersal and infection. Here we present the purification and characterization of spores from the environmental fungus Cryptococcus neoformans. For the first time, we purified spores to homogeneity and assessed their morphological, stress resistance, and surface properties. We found that spores are morphologically distinct from yeast cells and are covered with a thick spore coat. Spores are also more resistant to environmental stresses than yeast cells and display a spore-specific configuration of polysaccharides on their surfaces. Surprisingly, we found that the surface of the spore reacts with antibodies to the polysaccharide glucuronoxylomannan, the most abundant component of the polysaccharide capsule required for C. neoformans virulence. We explored the role of capsule polysaccharide in spore development by assessing spore formation in a series of acapsular strains and determined that capsule biosynthesis genes are required for proper sexual development and normal spore formation. Our findings suggest that C. neoformans spores may have an adapted cell surface that facilitates persistence in harsh environments and ultimately allows them to infect mammalian hosts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.