ArticleEukaryotic cell2009
HacA-dependent transcriptional switch releases hacA mRNA from a translational block upon endoplasmic reticulum stress.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila.Microbial biotechnology · 2025Article
- TheJournal of fungi (Basel, Switzerland) · 2024Article
- Fundamental and Applicative Aspects of the Unfolded Protein Response in Yeasts.Journal of fungi (Basel, Switzerland) · 2023Review
- Putative Core Transcription Factors Affecting Virulence inJournal of fungi (Basel, Switzerland) · 2023Article
- Systematic Characterization of bZIP Transcription Factors Required for Development and Aflatoxin Generation by High-Throughput Gene Knockout inJournal of fungi (Basel, Switzerland) · 2022Article
- Development of a versatile copper-responsive gene expression system in the plant-pathogenic fungus Fusarium graminearum.Molecular plant pathology · 2021Article
- Unfolded Protein Response and Scaffold Independent Pheromone MAP Kinase Signaling ControlJournal of fungi (Basel, Switzerland) · 2021Article
- Translation Control ofInternational journal of molecular sciences · 2019Review
- Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa.FEBS open bio · 2016Article
- System-wide characterization of bZIP transcription factor proteins involved in infection-related morphogenesis of Magnaporthe oryzae.Environmental microbiology · 2015Article
- Article
- Genome-wide analysis of the endoplasmic reticulum stress response during lignocellulase production in Neurospora crassa.Biotechnology for biofuels · 2015Article
- Polysome profiling reveals broad translatome remodeling during endoplasmic reticulum (ER) stress in the pathogenic fungus Aspergillus fumigatus.BMC genomics · 2014Article
- Review
- The fungal UPR: a regulatory hub for virulence traits in the mold pathogen Aspergillus fumigatus.Virulence · 2014Review
- Functional analysis of the Aspergillus nidulans kinome.PloS one · 2013Article
- Article
- HacA-independent functions of the ER stress sensor IreA synergize with the canonical UPR to influence virulence traits in Aspergillus fumigatus.PLoS pathogens · 2011Article
- Effects of a defective ERAD pathway on growth and heterologous protein production in Aspergillus niger.Applied microbiology and biotechnology · 2011Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Activation of the unfolded protein response (UPR) in eukaryotes involves the splicing of an unconventional intron from the mRNA encoding the transcriptional activator of the pathway. In Saccharomyces cerevisiae a 252-nucleotide (nt) unconventional intron is spliced out of the transcript of HAC1, changing the 3' end of the HAC1 open reading frame and relieving the transcript from a translational block in a single step. The translational block is caused by the base pairing of part of the unconventional intron with the 5'-untranslated region (5'UTR). In Aspergillus niger and other aspergilli, the unconventional intron in hacA mRNA is only 20 nt long. Since this intron is part of a stable stem-loop structure, base pairing with the 5'UTR, in contrast to the case with yeast HAC1, is not possible. However, analysis of the hacA mRNA revealed a GC-rich inverted repeat (18 base pairings). Upon the activation of the UPR, the 5'UTR of hacA mRNA is truncated by 230 nt, removing the left part of this inverted repeat. This implies a similar release of a translational block as in the case of S. cerevisiae HAC1 but in two steps. The mechanism behind the 5' truncation, which does not take place in either yeast HAC1 or mammalian xbp1 mRNA, has been hitherto unknown. Here we show that during secretion stress in A. niger, hacA transcription starts from a new start site closer to the ATG, relieving the transcript from translational attenuation. This transcriptional switch is mediated by HacA itself and the unfolded protein response element 2 (UPRE2) in the hacA promoter.
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