ArticleApplied and environmental microbiology2009
Improved fermentation performance of a lager yeast after repair of its AGT1 maltose and maltotriose transporter genes.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- Beer yeast breeding in the era of innovation: advances and applications for modern brewing.Biotechnology letters · 2025Review
- Expression patterns ofApplied and environmental microbiology · 2024Article
- Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain.Journal of fungi (Basel, Switzerland) · 2023Article
- Identification of European isolates of the lager yeast parent Saccharomyces eubayanus.FEMS yeast research · 2022Article
- Transcriptomic and metabolomic analysis reveals genes related to stress tolerance in high gravity brewing.World journal of microbiology & biotechnology · 2022Article
- Transcriptional Profile of the Industrial Hybrid Saccharomyces pastorianus Reveals Temperature-Dependent Allele Expression Bias and Preferential Orthologous Protein Assemblies.Molecular biology and evolution · 2021Article
- Packing a punch: understanding how flavours are produced in lager fermentations.FEMS yeast research · 2021Review
- HimalayanApplied and environmental microbiology · 2019Article
- Lager-brewing yeasts in the era of modern genetics.FEMS yeast research · 2019Review
- Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.PLoS genetics · 2019Article
- In vivo recombination of Saccharomyces eubayanus maltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation.PLoS genetics · 2019Article
- Laboratory Evolution of aFrontiers in genetics · 2019Article
- Quantitative Trait Nucleotides Impacting the Technological Performances of IndustrialFrontiers in genetics · 2019Article
- Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes inFrontiers in microbiology · 2018Article
- Maltose and maltotriose utilisation by group I strains of the hybrid lager yeast Saccharomyces pastorianus.FEMS yeast research · 2016Article
- Ploidy influences the functional attributes of de novo lager yeast hybrids.Applied microbiology and biotechnology · 2016Article
- New lager yeast strains generated by interspecific hybridization.Journal of industrial microbiology & biotechnology · 2015Article
- Lager yeast comes of age.Eukaryotic cell · 2014Review
- Influence of valine and other amino acids on total diacetyl and 2,3-pentanedione levels during fermentation of brewer's wort.Applied microbiology and biotechnology · 2013Article
- The temperature dependence of maltose transport in ale and lager strains of brewer's yeast.FEMS yeast research · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of more concentrated, so-called high-gravity and very-high-gravity (VHG) brewer's worts for the manufacture of beer has economic and environmental advantages. However, many current strains of brewer's yeasts ferment VHG worts slowly and incompletely, leaving undesirably large amounts of maltose and especially maltotriose in the final beers. alpha-Glucosides are transported into Saccharomyces yeasts by several transporters, including Agt1, which is a good carrier of both maltose and maltotriose. The AGT1 genes of brewer's ale yeast strains encode functional transporters, but the AGT1 genes of the lager strains studied contain a premature stop codon and do not encode functional transporters. In the present work, one or more copies of the AGT1 gene of a lager strain were repaired with DNA sequence from an ale strain and put under the control of a constitutive promoter. Compared to the untransformed strain, the transformants with repaired AGT1 had higher maltose transport activity, especially after growth on glucose (which represses endogenous alpha-glucoside transporter genes) and higher ratios of maltotriose transport activity to maltose transport activity. They fermented VHG (24 degrees Plato) wort faster and more completely, producing beers containing more ethanol and less residual maltose and maltotriose. The growth and sedimentation behaviors of the transformants were similar to those of the untransformed strain, as were the profiles of yeast-derived volatile aroma compounds in the beers.
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