ArticleJournal of bacteriology2009
Role of Clp proteins in expression of virulence properties of Streptococcus mutans.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 98 citations in OpenAlex.
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- Article
- MecA: A Multifunctional ClpP-Dependent and Independent Regulator in Gram-Positive Bacteria.Molecular microbiology · 2025Review
- MecA inmSphere · 2024Article
- A rare waterborne outbreak ofEmerging microbes & infections · 2024Article
- Genome-wide analysis of acid tolerance genes of Enterococcus faecalis with RNA-seq and Tn-seq.BMC genomics · 2024Article
- Diverse nature of ClpX degradation motifs inMicrobiology spectrum · 2024Article
- mltG gene deletion mitigated virulence potential of Streptococcus mutans: An in-vitro, ex-situ and in-vivo study.AMB Express · 2023Article
- Proteomic Characterization of Virulence Factors and Related Proteins inInternational journal of molecular sciences · 2022Article
- Persistence ofFoods (Basel, Switzerland) · 2022Article
- Clp ATPases differentially affect natural competence development in Streptococcus mutans.MicrobiologyOpen · 2022Article
- ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans.Journal of bacteriology · 2022Article
- Investigating CRISPR spacer targets and their impact on genomic diversification ofFrontiers in genetics · 2022Article
- The lack of the cell division protein FtsZ induced generation of giant cells under acidic stress in cyanobacterium Synechocystis sp. PCC6803.Photosynthesis research · 2021Article
- Proteomic Characterization of Antibiotic Resistance inInternational journal of molecular sciences · 2021Article
- Regulatory involvement of the PerR and SloR metalloregulators in theJournal of bacteriology · 2021Article
- Increased Oxidative Stress Tolerance of a Spontaneously OccurringJournal of bacteriology · 2021Article
- Characterization of FtsH Essentiality inFrontiers in genetics · 2021Article
- Significance of Individual Domains of ClpL: A Novel Chaperone fromBiochemistry · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Mutational analysis revealed that members of the Clp system, specifically the ClpL chaperone and the ClpXP proteolytic complex, modulate the expression of important virulence attributes of Streptococcus mutans. Compared to its parent, the DeltaclpL strain displayed an enhanced capacity to form biofilms in the presence of sucrose, had reduced viability, and was more sensitive to acid killing. The DeltaclpP and DeltaclpX strains displayed several phenotypes in common: slow growth, tendency to aggregate in culture, reduced autolysis, and reduced ability to grow under stress, including acidic pH. Unexpectedly, the DeltaclpP and DeltaclpX mutants were more resistant to acid killing and demonstrated enhanced viability in long-term survival assays. Biofilm formation by the DeltaclpP and DeltaclpX strains was impaired when grown in glucose but enhanced in sucrose. In an animal study, the average number of S. mutans colonies recovered from the teeth of rats infected with the DeltaclpP or DeltaclpX strain was slightly lower than that of the parent strain. In Bacillus subtilis, the accumulation of the Spx global regulator, a substrate of ClpXP, has accounted for the DeltaclpXP phenotypes. Searching the S. mutans genome, we identified two putative spx genes, designated spxA and spxB. The inactivation of either of these genes bypassed phenotypes of the clpP and clpX mutants. Western blotting demonstrated that Spx accumulates in the DeltaclpP and DeltaclpX strains. Our results reveal that the proteolysis of ClpL and ClpXP plays a role in the expression of key virulence traits of S. mutans and indicates that the underlying mechanisms by which ClpXP affect virulence traits are associated with the accumulation of two Spx orthologues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.