ArticleJournal of bacteriology2009
Roles of germination-specific lytic enzymes CwlJ and SleB in Bacillus anthracis.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- YpeB stability affects germination possibly through delaying SleB activity inJournal of bacteriology · 2026Article
- Structural and functional analysis ofMicrobiology (Reading, England) · 2025Article
- Review
- A Family of Spore Lipoproteins Stabilizes the Germination Apparatus by Altering Inner Spore Membrane Fluidity inJournal of bacteriology · 2023Article
- Suboptimal Bacillus licheniformis and Bacillus weihenstephanensis Spore Incubation Conditions Increase Heterogeneity of Spore Outgrowth Time.Applied and environmental microbiology · 2020Article
- YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores.BMC microbiology · 2019Article
- Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis spores.Microbial cell factories · 2019Article
- Peptidoglycan Muropeptides: Release, Perception, and Functions as Signaling Molecules.Frontiers in microbiology · 2019Review
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- Surviving Between Hosts: Sporulation and Transmission.Microbiology spectrum · 2016Review
- Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division.Acta biochimica et biophysica Sinica · 2016Article
- A Quality-Control Mechanism Removes Unfit Cells from a Population of Sporulating Bacteria.Developmental cell · 2015Article
- HtrC is involved in proteolysis of YpeB during germination of Bacillus anthracis and Bacillus subtilis spores.Journal of bacteriology · 2015Article
- Article
- Role of YpeB in cortex hydrolysis during germination of Bacillus anthracis spores.Journal of bacteriology · 2014Article
- Spore germination mediated by Bacillus megaterium QM B1551 SleL and YpeB.Journal of bacteriology · 2014Article
- Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.Journal of bacteriology · 2013Article
- Bacterial cell-wall recycling.Annals of the New York Academy of Sciences · 2013Review
- Article
- Crystal structure of the catalytic domain of the Bacillus cereus SleB protein, important in cortex peptidoglycan degradation during spore germination.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The structural characteristics of a spore enable it to withstand stresses that typically kill a vegetative cell. Spores remain dormant until small molecule signals induce them to germinate into vegetative bacilli. Germination requires degradation of the thick cortical peptidoglycan by germination-specific lytic enzymes (GSLEs). Bacillus anthracis has four putative GSLEs, based upon sequence similarities with enzymes in other species: SleB, CwlJ1, CwlJ2, and SleL. In this study, the roles of SleB, CwlJ1, and CwlJ2 were examined. The expression levels of all three genes peak 3.5 h into sporulation. Genetic analysis revealed that, similar to other known GSLEs, none of these gene products are individually required for growth, sporulation, or triggering of germination. However, later germination events are affected in spores lacking CwlJ1 or SleB. Compared to the wild type, germinating spores without CwlJ1 suffer a delay in optical density loss and cortex peptidoglycan release. The absence of SleB also causes a delay in cortex fragment release. A double mutant lacking both SleB and CwlJ1 is completely blocked in cortex hydrolysis and progresses through outgrowth to produce colonies at a frequency 1,000-fold lower than that of the wild-type strain. A null mutation eliminating CwlJ2 has no effect on germination. High-performance liquid chromatography and mass spectroscopy analysis revealed that SleB is required for lytic transglycosylase activity. CwlJ1 also clearly participates in cortex hydrolysis, but its specific mode of action remains unclear. Understanding the lytic germination activities that naturally diminish spore resistance can lead to methods for prematurely inducing them, thus simplifying the process of treating contaminated sites.
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