ArticleJournal of bacteriology2009
Pathway of gamma-aminobutyrate metabolism in Rhizobium leguminosarum 3841 and its role in symbiosis.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- Polyhydroxyalkanoate synthesis by Sinorhizobium meliloti drives a host-specific collapse in symbiosis with Medicago sativa.Research square · 2025Article
- Enhanced accumulation of γ-aminobutyric acid by deletion of aminotransferase genes involved in γ-aminobutyric acid catabolism in engineeredApplied and environmental microbiology · 2024Article
- Identification and functional analysis of recent IS transposition events in rhizobia.Mobile DNA · 2024Article
- Aspartate aminotransferase ofMicrobiology (Reading, England) · 2024Article
- Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15).BMC microbiology · 2022Article
- The Proteogenome of SymbioticMicroorganisms · 2022Article
- Deciphering Genomes: Genetic Signatures of Plant-AssociatedFrontiers in plant science · 2022Article
- Article
- Metabolic control of nitrogen fixation in rhizobium-legume symbioses.Science advances · 2021Article
- How Rhizobia Adapt to the Nodule Environment.Journal of bacteriology · 2021Article
- Antioxidant ability of glutaredoxins and their role in symbiotic nitrogen fixation inApplied and environmental microbiology · 2021Article
- Article
- Responses of mature symbiotic nodules to the whole-plant systemic nitrogen signaling.Journal of experimental botany · 2020Article
- Glutathione is Involved in Detoxification of Peroxide and Root Nodule Symbiosis of Mesorhizobium huakuii.Current microbiology · 2020Article
- A GMC Oxidoreductase GmcA Is Required for Symbiotic Nitrogen Fixation inFrontiers in microbiology · 2020Article
- Antioxidation and symbiotic nitrogen fixation function of prxA gene in Mesorhizobium huakuii.MicrobiologyOpen · 2019Article
- From Intracellular Bacteria to Differentiated Bacteroids: Transcriptome and Metabolome Analysis inJournal of bacteriology · 2019Article
- Diverse role of γ-aminobutyric acid in dynamic plant cell responses.Plant cell reports · 2019Review
- Transcriptomic analysis of Rhizobium leguminosarum bacteroids in determinate and indeterminate nodules.Microbial genomics · 2019Article
- Genome-Wide Association Analyses in the Model RhizobiummSphere · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Pea plants incubated in 15N2 rapidly accumulated labeled gamma-aminobutyrate (GABA) in the plant cytosol and in bacteroids of Rhizobium leguminosarum bv. viciae 3841. Two pathways of GABA metabolism were identified in R. leguminosarum 3841. In the first, glutamate is formed by GABA aminotransferase (GabT), transferring the amino group from GABA to 2-oxoglutarate. In the second, alanine is formed by two omega-aminotransferases (OpaA and OpaB), transferring the amino group from GABA to pyruvate. While the gabT mutant and the gabT opaA double mutant grew on GABA as a nitrogen source, the final triple mutant did not. The semialdehyde released from GABA by transamination is oxidized by succinate semialdehyde dehydrogenase (GabD). Five of six potential GabD proteins in R. leguminosarum bv. viciae 3841 (GabD1, -D2, -D3, -D4, and -D5) were shown by expression analysis to have this activity. However, only mutations of GabD1, GabD2, and GabD4 were required to prevent utilization of GABA as the sole nitrogen source in culture. The specific enzyme activities of GabT, Opa, and GabD were highly elevated in bacteroids relative to cultured bacteria. This was due to elevated expression of gabT, opaA, gabD1, and gabD2 in nodules. Strains mutated in aminotransferase and succinate semialdehyde dehydrogenases (gabT, opaA, or opaB and gabD1, gabD2, or gabD4, respectively) that cannot use GABA in culture still fixed nitrogen on plants. While GABA catabolism alone is not essential for N2 fixation in bacteroids, it may have a role in energy generation and in bypassing the decarboxylating arm of the tricarboxylic acid cycle.
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