ArticleNucleic acids research2009
DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- A method for targeting a specified segment of DNA to a bacterial microorganelle.Nucleic acids research · 2022Article
- Article
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- Experimental approaches to identify small RNAs and their diverse roles in bacteria--what we have learnt in one decade of MicA research.MicrobiologyOpen · 2015Review
- Silencing of DNase Colicin E8 Gene Expression by a Complex Nucleoprotein Assembly Ensures Timely Colicin Induction.PLoS genetics · 2015Article
- "Non-canonical protein-DNA interactions identified by ChIP are not artifacts": response.BMC genomics · 2013Article
- Location and dynamics of an active promoter in Escherichia coli K-12.The Biochemical journal · 2012Article
- To understand the whole, you must know the parts: unraveling the roles of protein-DNA interactions in genome regulation.The Analyst · 2011Review
- Chromosomal macrodomains and associated proteins: implications for DNA organization and replication in gram negative bacteria.PLoS genetics · 2011Review
- Sequence-specific capture of protein-DNA complexes for mass spectrometric protein identification.PloS one · 2011Article
- Advantages and limitations of current network inference methods.Nature reviews. Microbiology · 2010Review
- Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site.Journal of bacteriology · 2010Article
- Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains.BMC microbiology · 2009Article
- DNA damage-signaling, homologous recombination and genetic mutation induced by 5-azacytidine and DNA-protein crosslinks in Escherichia coli.Mutation researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.